Comparative on Peptide Content in Pre-and-Post-Processed Trionycis Carapax

HAN Qiu-jun; BI Wei; Wang Wei; LI Qu-sheng; WANG Peng-long; XU Shi-xun; WANG Yan-hui; XU Kuo; WANG Lin; CHENG Ya-tao; LI Qiang; LEI Hai-min

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Comparative on Peptide Content in Pre-and-Post-Processed Trionycis Carapax

更新时间：2024-01-04

- Comparative on Peptide Content in Pre-and-Post-Processed Trionycis Carapax
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 24, Pages: 86-88(2012)
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- Published：2012
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HAN Qiu-jun, BI Wei, Wang Wei, et al. Comparative on Peptide Content in Pre-and-Post-Processed Trionycis Carapax[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(24): 86-88.
DOI：

HAN Qiu-jun, BI Wei, Wang Wei, et al. Comparative on Peptide Content in Pre-and-Post-Processed Trionycis Carapax[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(24): 86-88. DOI：

摘要

目的: 建立双缩脲反应-酶联免疫检测仪对肽类的快速定量方法

测定鳖甲炮制前后肽类含量差异

探讨鳖甲炮制科学性。方法: 应用0.02 g·mL-1 酒石酸钠钾50 μL

5%氢氧化钠20 μL

0.5%硫酸铜50 μL的双缩脲显色剂测定鳖甲炮制前后肽类含量

检测波长580 nm。结果: 鳖甲七肽在0.10~5.00 g·L-1 与吸光度呈良好的线性关系

醋鳖甲平均总肽含量为6.99%

生鳖甲平均总肽含量为1.04%。结论: 醋鳖甲总肽含量明显高于生鳖甲总肽含量

醋制法可提高鳖甲有效成分溶出度。

Abstract

Objective:A rapid method was established for quantitative determination of peptide based on biuret reaction-enzyme-linked immunosorbent detector

and peptide content in the same geographical origin of pre-and-post-processed Trionycis Carapax was analyzed. Method: The developer was 0.02 g·mL-1 sodium potassium tartrate solution (50 μL)

5% NaOH (20 μL)

1% CuSO4·5H2O (50 μL). The wavelength of detection was at 580 nm. Result: The calibration curves were linear in the ranges of 0.00-5.00 g·L-1 for septa-peptide from Trionycis Carapax. Peptide content in vinegar-processed samples was 6.99%

while the same content in crud samples was 1.04%. Conclusion: Peptide content in vinegar-processed samples was significantly higher than the one in crud samples. The content of Trionycis Carapax is increased greatly after vinegar by decoction.

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Related Author

DUAN Haohan

JIA Hao

LEI Jingwei

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XIE Caixia

GONG Haiyan

ZHU Beibei

WANG Yanjing

Related Institution

Henan Engineering Research Center for Quality Control and Evaluation of Traditional Chinese Medicine（TCM），School of Pharmacy，Henan University of Chinese Medicine

Luohe Medical College

Third Affiliated Hospital of Henan University of Chinese Medicine

Tianjin University of Traditional Chinese Medicine

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

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