Objective:To establish an HPLC-DAD-ELSD method for the simultaneous determination of spinosin

jujuboside A

jujuboside B and betulinic acid in Semen Ziziphi Spinosa dispersible tablet. Method: The chromatographic separation was performed on a Venusil XBP reversed phase C18 (4.6 mm×250 mm

5 μm) analytical column by a gradient elution program with acetonitrile-water (containing 0.1% acetic acid) at a flow rate of 1.0 mL·min-1.The column temperature was at 25 ℃ and the optimum detection wavelength of DAD was set at 335 nm

the evaporator tube temperature of ELSD was set at 100 ℃ with the nebulizing gas flow rate of 2.9 L·-1. Result: The linear range of spinosin

jujuboside A

jujuboside B and betulinic acid were 3.36-32.36(r=0.999 2)

1.12-11.24(r=0.999 6)

1.36-13.56(r=0.999 7) 5.04-50.45 μg·g-1 (r=0.999 3)

respectively. The average recoveries were 99.23% (RSD 1.51%)

101.25 % (RSD 1.49%)

98.63% (RSD 2.57%)

102.09% (RSD 0.72%)

respectively. Conclusion: The method is simple and accurate

it can be used for the quality control of Semen Ziziphi Spinosae dispersible tablet.