Optimization of Extraction Technology for Effective Parts from  by Orthogonal Test

MENG Mei; WANG Hua-yu; REN Yu; ZHANG Wei-yu

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Optimization of Extraction Technology for Effective Parts from by Orthogonal Test

更新时间：2024-01-04

- Optimization of Extraction Technology for Effective Parts from by Orthogonal Test
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 6, Pages: 46-49(2013)
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- Published：2013
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MENG Mei, WANG Hua-yu, REN Yu, et al. Optimization of Extraction Technology for Effective Parts from by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(6): 46-49.
DOI：

MENG Mei, WANG Hua-yu, REN Yu, et al. Optimization of Extraction Technology for Effective Parts from by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(6): 46-49. DOI：

摘要

目的: 优选山豆根有效部位的提取工艺。方法: 采用L9(34)正交试验法

以总生物碱含量为指标

选取乙醇体积分数、加醇量、提取时间及提取次数为考察因素

优选山豆根中总生物碱的提取工艺;以总多糖含量为指标

选取加水倍量、提取时间及提取次数为考察因素

优选山豆根中总多糖的提取工艺。结果: 总生物碱最佳提取工艺为加6倍量70%乙醇提取2次

每次1 h;总多糖最佳提取工艺为加6倍量水提取3次

每次1 h。结论: 优选的提取工艺操作简单、稳定可行

适合于产业化生产

具有良好的应用前景。

Abstract

Objective: To optimize extraction technology of effective parts from Sophora tonkinensis. Method: Taking the content of total alkaloids as index

with extraction time

extraction times

ethanol concentration and amount as factors

extraction technology of total alkaloids from S.tonkinensis was optimized by L9(34) orthogonal test;With the content of total polysaccharides as index

taking the amount of water

extraction time and times as factors

extraction technology of total polysaccharides was optimized by orthogonal test. Result: Optimal extraction technology of total alkaloids was as following:extracted 2 times with 6 times the amount of 70% ethanol

1 h per time;Optimum extraction technology of total polysaccharides was as following:extracted 3 times with 6 times the amount of water

1.0 h each time. Conclusion: This optimized extraction technology was stable and feasible

it was suitable for industrial production with a good prospect.

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Related Author

BAO Ju-tai

WEI Ling-yu

LIU Qian

LI Zhi-hui

LIU Xiao-ya

CAO Yan-ling

XIE Fei

WANG Wei

Related Institution

College of Traditional Chinese Medicine,Hebei United University

School of Chinese Materia Medica,Beijing University of Traditional Chinese Medicine

Research Center for Life Science and Environmental Sciences,Haerbin University of Commerce,Haerbin

Qiannan Medical College for Nationalities,Duyun

Guiyang College of Traditional Chinese Medicine

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