Molecular Mechanism of Cinnamon Polyphenols on Improvement of Insulin Resistance in HepG2 Cells

LU Zhao-lian; HUANG Cai-guo

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Molecular Mechanism of Cinnamon Polyphenols on Improvement of Insulin Resistance in HepG2 Cells

更新时间：2024-01-04

- Molecular Mechanism of Cinnamon Polyphenols on Improvement of Insulin Resistance in HepG2 Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 24, Pages: 276-279(2012)
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- Published：2012
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LU Zhao-lian, HUANG Cai-guo. Molecular Mechanism of Cinnamon Polyphenols on Improvement of Insulin Resistance in HepG2 Cells [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(24): 276-279.
DOI：

LU Zhao-lian, HUANG Cai-guo. Molecular Mechanism of Cinnamon Polyphenols on Improvement of Insulin Resistance in HepG2 Cells [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(24): 276-279. DOI：

摘要

目的: 研究肉桂多酚改善HepG2 细胞胰岛素抵抗的分子机制。方法: 以HepG2细胞为模型

设立对照组和肉桂多酚实验组

肉桂多酚组设5

15 mg·L-1 3种不同质量浓度组。肉桂多酚作用细胞24 h后

运用实时定量聚合酶链反应(RT-PCR)技术

检测肉桂多酚对胰岛素抵抗HepG2细胞内葡萄糖转运蛋白2(GLUT2) 、磷酸烯醇丙酮酸羧激酶(PEPCK)和葡萄糖-6-磷酸酶(G-6-Pase)基因表达的影响。结果: 与对照组比较

肉桂多酚作用细胞24 h后

降低了GLUT2 mRNA的表达(P<0.05);肉桂多酚能够明显降低PEPCK

G-6-Pase mRNA的表达(P<0.05

P<0.01)

且随着浓度的升高

肉桂多酚对PEPCK和G-6-Pase mRNA表达的抑制作用越明显。结论: 肉桂多酚对HepG2 胰岛素抵抗具有明显的改善作用

其机制可能与降低细胞内GLUT2

PEPCK和G-6-Pase mRNA的表达有关。

Abstract

Objective:To study the molecular mechanism of cinnamon polyphenols on improvement of insulin resistance in HepG2 cells in vitro. Method: The HepG2 cells were used as cell model. Cells were incubated with cinnamon polyphenols of three different concentrations (5

15 mg·L-1).Control cells were incubated without cinnamon polyphenols. After 24 h

the mRNA expression of porcine glucose transporter 2 (GLUT2) phosphoenolpyruvate carboxykinase(PEPCK) and glucose-6-phosphatase (G-6-Pase) related to insulin resistance was detected by Real-time PCR(RT-PCR). Result: Compared with the control group

the RT-PCR showed the mRNA expression of GLUT2 decreased after being treated with the cinnamon polyphenols(P<0.05);the cinnamon polyphenols significantly decreased the expression of PEPCK and G-6-Pase mRNA(P<0.05

P<0.01)

moreover

the cinnamon polyphenols inhibited the expression of PEPCK and G-6-Pase mRNA in a dose-dependent manner. Conclusion: Cinnamon polyphenols can significantly improve insulin-resistance of HepG2 cells and its mechanism is related to the inhibition of GLUT2

PEPCK

G-6-Pase gene expression.

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