Determination of Chlorogenic Acid in by High Performance Liquid Chromatography
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Determination of Chlorogenic Acid in by High Performance Liquid Chromatography
Chinese Journal of Experimental Traditional Medical FormulaeVol. 19, Issue 4, Pages: 99-102(2013)
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Published:2013
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DING Yue, ZHANG Yong, DING Wen-ping, et al. Determination of Chlorogenic Acid in by High Performance Liquid Chromatography[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(4): 99-102.
DOI:
DING Yue, ZHANG Yong, DING Wen-ping, et al. Determination of Chlorogenic Acid in by High Performance Liquid Chromatography[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(4): 99-102.DOI:
Determination of Chlorogenic Acid in by High Performance Liquid Chromatography
Objective: To establish the determination method of chlorogenic acid in Sargentodoxa cuneata. Method: HPLC was used with Diamonsil ODS-C18 column (4.6 mm×250 mm
5 μm) as chromatographic column and acetonitrile-4% phosphoric acid solution (11:89) as mobile phase. The flow rate was 1.0 mL·min-1; the detection wavelength was set at 327 nm and the column temperature was kept at 25℃. Result: The linear range of chlorogenic acid was 0.000 102-0.010 2 mg. The average recovery was 102.57%
RSD 0.83% (n=6). Conclusion: The method of sample processing was simple and the HPLC method was accurate and reliable. It can be used for the quality control of S. cuneata and its preparation.