Objective:To develop a new method for simultaneous determination of daucosterol and β-sitosterol in Alysicarpus vaginalis. Method: HPLC-ELSD method was used to determine daucosterol and β-sitosterol. The separation was performed on a Diamonsil C18 column (4.6 mm×150 mm

5 μm) with methanol-water (95:5) as the mobile phase at the flow rate of 1.0 mL·min-1. The temperature in drift tube was 80℃ and the carrier gas flow was 2.5 L·min-1. Result: The linear ranges of daucosterol and β-sitosterol were 10-100 μg·mL-1 (r=0.999 3) and 25-250 mg·L-1 (r=0.999 1)

respectively. The average recoveries were 99.5%

98.9%

and RSDs were 2.4% and 2.1%

respectively. Conclusion: This method is selective

simple

accurate

which can be applied for simultaneous determination of daucosterol and β-sitosterol in A. vaginalis.