Objective: To study factors affecting stability of β-CD inclusion compound of volatile oil from traditional Chinese medicine

and compare stability difference from inclusion compounds which prepared by two methods. Method: With relative volatile oil loss rate as index

single factor test was used to explore stability under these conditions

such as temperature of 60 ℃

light of (4 500± 500) lx and humidity of 75%.Volatile oil in inclusion compound was extracted by steam distillation method

composition change was detected by GC-MS between before and after stability test. Result: After inclusion of volatile oil

its stability to light

heat and humidity had significantly improved

3 kinds of factors affecting relative volatile oil loss rate were in order of high humidity>high temperature>strong light;Three factors had greater effect on composition in volatile oil inclusion with heavy gravity and high refractive index than that of small gravity and low refractive index. Conclusion: Inclusion prepared by colloid mill method has better stability on volatile oil content and composition than saturated aqueous solution method.