Studies of HPLC Characteristic Chromatogramof Saponins in Effective Parts of Xuechangning Formula

LIN Hui; RAO Jian-hua; PAN Yi; YANG Tao; XU Da-liang; ZHANG Peng

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Studies of HPLC Characteristic Chromatogramof Saponins in Effective Parts of Xuechangning Formula

更新时间：2024-01-04

- Studies of HPLC Characteristic Chromatogramof Saponins in Effective Parts of Xuechangning Formula
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 6, Pages: 127-130(2013)
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- Published：2013
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LIN Hui, RAO Jian-hua, PAN Yi, et al. Studies of HPLC Characteristic Chromatogramof Saponins in Effective Parts of Xuechangning Formula[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(6): 127-130.
DOI：

LIN Hui, RAO Jian-hua, PAN Yi, et al. Studies of HPLC Characteristic Chromatogramof Saponins in Effective Parts of Xuechangning Formula[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(6): 127-130. DOI：

摘要

目的: 建立血畅宁组方有效部位总皂苷的HPLC特征图谱。方法: 采用HPLC法

色谱条件Synergi Fusion-RP C18(4.6 mm×250 mm

5 μm);流动相为乙腈-水溶液

梯度洗脱进行色谱分离;检测波长 203 nm;流速1.0 mL·min-1;柱温(30±5) ℃。结果: 12批不同批次的血畅宁组方药材所得皂苷部位中

除3批外

其余9批与系统生成的对照特征图谱的相似度均在0.9以上;归纳出血畅宁组方皂苷部位有19个共有峰

不同批次样品中主要化学成分组成相似

但相对比例有明显的差异。结论: 所建立的方法简单可行

能有效控制血畅宁组方有效部位总皂苷的质量。

Abstract

Objective: To study the characteristic chromatogram of saponins in effective parts of Xuechangning formula by HPLC. Method: The chromatographic condition of HPLC was as follows: Synergi Fusion-RP column C18(4.6 mm×250 mm

5 μm) at 30 ℃

the mobile phase being acetonitrile with water

gradient elution in chromatographic separation

the detective wavelength being 203 nm

and flow rate being 1.0 mL·min-1. Result: Except for three batches of 12 batches of saponins in Xuechangning formula

the characteristic chromatogram similarity value of the other eight batches was above 0.9 as compared with system generation spectrum

and 19 common characteristic peaks were presented.The results showed that the main chemical constituents of medicinal materials were similar among samples from different habitats

but their relative ratios are significantly different. Conclusion: The method is simple

accurate and reproducibility

and can be used for the quality control of Xuechangning formula.

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