Determination of Chlorogenic Acid in Cangling Zhixie Oral Solution by HPLC
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Determination of Chlorogenic Acid in Cangling Zhixie Oral Solution by HPLC
Chinese Journal of Experimental Traditional Medical FormulaeVol. 19, Issue 2, Pages: 121-123(2013)
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Published:2013
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LI Xiang, LIU Gui-yang, MA Jian-li, et al. Determination of Chlorogenic Acid in Cangling Zhixie Oral Solution by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(2): 121-123.
DOI:
LI Xiang, LIU Gui-yang, MA Jian-li, et al. Determination of Chlorogenic Acid in Cangling Zhixie Oral Solution by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(2): 121-123.DOI:
Determination of Chlorogenic Acid in Cangling Zhixie Oral Solution by HPLC
Objective: To establish an HPLC method for determination of chlorogenic acid in Cangling Zhixie oral solution. Method: The Agilent Eclipse XDB-C18 was used with the mobile phase consisted of methanol-0.5% acetic acid (20: 80); the flow rate was 1.0 mL· min-1; the detection wavelength was at 327 nm
and the column temperature was at room temperature. Result: A good linear correlation of chlorogenic acid was observed within the range of 0.021 84-0.436 8 μg. The average recovery was 100.97% with RSD 2.10% (n=6). Conclusion: The method is simple
rapid
accurate and reliable. It can be used for the determination of chlorogenic acid in Cangling Zhixie oral solution.