Determination of Allantoin in Lithospermun Zollingeri by HPLC

XU Zhi-hong; JIA Xiao-bin; CHEN Lei-yao

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Determination of Allantoin in Lithospermun Zollingeri by HPLC

更新时间：2024-01-04

- Determination of Allantoin in Lithospermun Zollingeri by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 23, Pages: 112-114(2012)
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- Published：2012
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XU Zhi-hong, JIA Xiao-bin, CHEN Lei-yao. Determination of Allantoin in Lithospermun Zollingeri by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(23): 112-114.
DOI：

XU Zhi-hong, JIA Xiao-bin, CHEN Lei-yao. Determination of Allantoin in Lithospermun Zollingeri by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(23): 112-114. DOI：

摘要

目的: 建立梓木草饮片中尿囊素含量测定的方法。方法: 采用HPLC测定。色谱柱Hypersil ODS-C18柱(4.6 mm×250 mm

5 μm)

流动相甲醇-水(5∶95)

流速0.2 mL·min-1

检测波长210 nm

柱温30 ℃

进样量20 μL。结果: 尿囊素在12.32~61.60 μg呈良好的线性关系(r=0.999 9);尿囊素平均加样回收率为98.1%

RSD 1.55%(n=6)。结论: 该方法可以作为梓木草饮片的质量控制

不同产地梓木草饮片中尿囊素含量差异较大。

Abstract

Objective: To establish a method for the determination of allantoin in Lithospermun zollingeri. Method: Separation and determination of allantoin were accessed by using an ODS-C18 column and a mobile phase of methanol-water at a ratio of 5∶95. The flow rate was 0.2 mL·min-1

temperature at 30 ℃ and detection wavelength at 210 nm. Result: A good linearity of allantoin was achieved in the range of 12.32-61.60 μg(r=0.999 9).The mean value of recovery was 98.1%

RSD was 1.55%. Conclusion: The method in this paper could provide an evidence for quality control of L. zollingeri and there is comparatively large difference of content of allantoin in L. zollingeri from different producing areas.

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Related Institution

Henan Engineering Research Center for Quality Control and Evaluation of Traditional Chinese Medicine（TCM），School of Pharmacy，Henan University of Chinese Medicine

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Third Affiliated Hospital of Henan University of Chinese Medicine

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Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

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