Determination of Paeniflorin Content in Lidan Zhitong Capsules by HPLC

WANG Ming-xing; YU Xiu-hua; LIU Yong-qiang

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Determination of Paeniflorin Content in Lidan Zhitong Capsules by HPLC

更新时间：2024-01-04

- Determination of Paeniflorin Content in Lidan Zhitong Capsules by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 22, Pages: 142-144(2012)
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- Published：2012
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WANG Ming-xing, YU Xiu-hua, LIU Yong-qiang. Determination of Paeniflorin Content in Lidan Zhitong Capsules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(22): 142-144.
DOI：

WANG Ming-xing, YU Xiu-hua, LIU Yong-qiang. Determination of Paeniflorin Content in Lidan Zhitong Capsules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(22): 142-144. DOI：

摘要

目的: 提高利胆止痛胶囊质量标准

增加含量测定项以对药品进行更好的质量控制。方法: 采用HPLC对制剂方中赤芍进行含量测定。安捷伦Agilent Extend-C18(4 mm×250 mm

5 μm)色谱柱

流动相乙腈-0.1%磷酸溶液(15∶85)

检测波长230 nm

流速1 mL·min-1

理论板数按芍药苷峰计算应不低于4 000。结果: 芍药苷在0.144~0.720 μg呈良好的线性关系

精密度试验RSD 0.54%

样品在24 h内基本稳定(RSD 1.17%)

重复性试验RSD 1.48%

平均加样回收率为97.77%(RSD 1.48%)。依正文方法进行了10批样品的含量测定

利胆止痛胶囊芍药苷的平均含量0.772 0 mg/粒。结论: 该方法专属性强

灵敏度高、重复性好

可用于该制剂的质量控制。

Abstract

Objective:To establish a HPLC method for determining paeoniflorin content in Lidan Zhitong capsules. Method: The content of paeoniflorin from Radix paeoniad rubra was idetermined by HPLC. The Agilent Extend-C18 column (4 mm×250 mm

5 μm) was used with acetonitrile-0.1% phosphoric acid solution (15∶85) as mobile phase; the detection wavelength was set at 230 nm; flow rate was 1 mL·min-1. Number of theoretical plates by paeoniflorin peak calculation should not be less than 4 000. Result: The calibration curve of paeoniflorin was linear in the range of 0.144-0.720 μg.RSD of precision was 0.54%. RSD of stability was 1.17%. RSD of reproducibility was 1.48%. The average recovery was 97.77% with RSD of 1.48%. Conclusion: The method is selective and sensitive

can be used for quality of control of Lidan Zhitong capsule.

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Related Institution

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