Determination of Caffeic Acid and Rosmarinic Acid in  from Different Species and Different Habitat

ZHU De-quan; HUANG Song; CHEN Jian-nan; TAN Yu-lian; QU Ying-ying; ZHUANG Yan-jie

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Determination of Caffeic Acid and Rosmarinic Acid in from Different Species and Different Habitat

更新时间：2024-01-04

- Determination of Caffeic Acid and Rosmarinic Acid in from Different Species and Different Habitat
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 2, Pages: 114-117(2013)
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- Published：2013
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ZHU De-quan, HUANG Song, CHEN Jian-nan, et al. Determination of Caffeic Acid and Rosmarinic Acid in from Different Species and Different Habitat[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(2): 114-117.
DOI：

ZHU De-quan, HUANG Song, CHEN Jian-nan, et al. Determination of Caffeic Acid and Rosmarinic Acid in from Different Species and Different Habitat[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(2): 114-117. DOI：

摘要

目的: 测定不同品种、不同产地溪黄草咖啡酸与迷迭香酸的含量。方法: 采用HPLC

Dikma Diamonsil (2)(C18 4.6 mm×250 mm

5 μm)色谱柱

以乙腈-0.3%磷酸为流动相梯度洗脱

流速1.0 mL·min-1

柱温室温

检测波长 329 nm。结果: 咖啡酸和迷迭香酸的线性范围分别为0.045 4~0.908 0 μg(r=0.999 6)和0.219 2~4.384 0 μg(r=0.999 9)

平均回收率(n=5)分别为97.33% (RSD 2.20%)

103.32%(RSD 1.84%)。不同品种、不同产地溪黄草药材中咖啡酸、迷迭香酸的含量差异较大

其中纤花变种的溪黄草两者的平均含量最高。结论: 该方法简单快捷

适合于溪黄草中酚酸成分的含量测定研究

为溪黄草的质量控制提供了依据。

Abstract

Objective: To establish a HPLC method for the determination of caffeic acid and rosmarinic acid in Rabdosia serra from different species and different habitat. Method: HPLC method was performed on a Dikma Diamonsil(2) (C18 4.6 mm×250 mm

5 μm) with a mobile phase of methanol-0.3% phosphoric acid as mobile phase by gradient elution. The flow rate was 1.0 mL· min-1

column temperature was kept at 25℃ and detection wavelength was set at 329 nm. Result: Caffeic acid and rosmarinic acid showed good linearity in the ranges of 0.045 4-0.908 0 μg (r=0.999 6) and 0.219 2-4.384 0 μg(r=0.999 9 with average recoveries of 98.7% and 103.0%

respectively. The content of caffeic acid and rosmarinic acid in Rabdosia serra from different species and different habitat

and the content of both in R. lophanthoides was the highest. Conclusion: The method is simple

rapid for determination the content of caffeic acid and rosmarinic acid in R. serra

and can be used as the quality control of I. lophanthoides.

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Related Author

CHEN Ling-ling

CAI Yue-e

HUANG Song

CHEN Jian-nan

Wen-hua ZHENG

Ya-ya XI

Cong-cong QU

Hui-fen LI

Related Institution

Shandong University of Traditional Chinese Medicine

Liaoning University of Traditional Chinese Medicine

Institute of Traditional Chinese Medicine， Heilongjiang University of Chinese Medicine

Graduate School，Heilongjiang University of Chinese Medicine

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

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