Investigation of Different Extraction Technology for Addition and Subtraction Prescription of Huoluo Xiaoling Dan by Fingerprint

GAO Wen-juan; TAN Xue-mei; DAI Rong-hua; BI Kai-shun; CHEN Xiao-hui

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Investigation of Different Extraction Technology for Addition and Subtraction Prescription of Huoluo Xiaoling Dan by Fingerprint

更新时间：2024-01-04

- Investigation of Different Extraction Technology for Addition and Subtraction Prescription of Huoluo Xiaoling Dan by Fingerprint
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 6, Pages: 40-43(2013)
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- Published：2013
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GAO Wen-juan, TAN Xue-mei, DAI Rong-hua, et al. Investigation of Different Extraction Technology for Addition and Subtraction Prescription of Huoluo Xiaoling Dan by Fingerprint[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(6): 40-43.
DOI：

GAO Wen-juan, TAN Xue-mei, DAI Rong-hua, et al. Investigation of Different Extraction Technology for Addition and Subtraction Prescription of Huoluo Xiaoling Dan by Fingerprint[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(6): 40-43. DOI：

摘要

目的: 采用HPLC指纹图谱研究不同提取工艺对活络效灵丹加减方(HLXL)中化学成分变化的影响。方法: 以獐牙菜苦苷、芍药苷、隐丹参酮和异欧前胡素等11个特征指纹峰为指标

采用HPLC分析测定

条件为YMC-Pack ODS-A色谱柱

流动相甲醇-水梯度洗脱

检测波长210 nm

流速0.8 mL·min-1

柱温30 ℃。系统考察不同溶剂(水、乙醇、丙酮)、不同提取方式(超声、回流、冷浸)及单提混合液和混提液对各特征指纹峰相对含量的影响。结果: 不同工艺提取物中特征峰相对峰面积存在较大差异。溶剂种类和提取方式对HLXL成分的提取有较大影响。结论: 该方法重复性、精密度、稳定性良好

可用于HLXL不同提取工艺的指纹图谱研究。

Abstract

Objective: To study influence of different extraction technology on chemical compositions changes from addition and subtraction prescription of Huoluo Xiaoling Dan(HLXL) by HPLC fingerprint. Method: With swertiamarin

paeoniflorin

cryptotanshinone

isoimperatorin and other seven characteristic fingerprint peaks as index

which were determined by HPLC

with YMC-Pack ODS-A column

methonal-water(gradient elution) as mobile phase

detection wavelength 210 nm

flow rate 0.8 mL·min-1

column temperature 30 ℃.Effects of different solvents(water

ethanol

acetone)

extract models(ultrasound

refluxing

macerating) and mixed-extract and separated-extract on relative content of each characteristic peaks were systematically investigated. Result: Different technology extracts had significant difference on relative peak area of eleven evaluating indexes.Kinds of solvent and extraction types and order of mixing had a great influence upon extraction efficiency of chemical compositions from HLXL. Conclusion: This method was reproducible

precise

stable and could be applied to study on fingerprint of HLXL with different extraction technology.

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Related Institution

Anhui University of Traditional Chinese Medicine

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