Objective:To explore the effect of mulberry leaves polysaccharide on the immune function of mice with long-term heavy training. Method: One hundred and twenty mice were divided into six groups at random: quiet control group

training group

saline movement group

high dosage mulberry leaves polysaccharide movement group

medium dosage mulberry leaves polysaccharide movement group(250

200

150 mg·kg-1·d-1)

low dosage mulberry leaves polysaccharide movement group

with 20 mice in every group. In addition to quiet control group

other groups of mice do swimming training with 5% weight for 50 minutes

with 6 times a week. The mice in mulberry leaves polysaccharide movement group were orally given with the dosage of 150

200

250 mg·kg-1·d-1 while the mice in saline movement group were given with normal saline for 4 weeks.The hemogram

body weight

phagocytic function of macrophages

index of spleen and thymus. serum agglutination titer

the quantity of plaque-forming cell were tested. Result: Compared with the saline movement group

there was a significant increase in WBC

Lym absolute value

absolute value of neutrophilic granulocyte

mulberry leaves polysaccharid could increase phagocytic percentage and phagocytic index of mice peritoneal macrophage

which was significantly higher in phagocytic percentage of mulberry leaves polysaccharid medium high dose group(35.7±5.17)%

(36.7±4.83)% than that in the saline movement group (29.2±4.27)% and was significantly higher in phagocytic index of mulberry leaves polysaccharid medium high dose group(3.19±1.23

2.49±1.29) than that in the saline movement group(3.91±1.29)(P<0.05). polysaccharid could increase the weight of thymus and spleen significantly(P<0.05)

and could increase index of spleen and thymus. Mulberry Leaves polysaccharid could increase serum agglutination titer and the quantity of plaque-forming cell significantly(P<0.05-P<0.01). Concllusion: Mulberry leaves polysaccharides can enhance long-term heavy load exercise mice immune function

regulation of immune cells and immune molecules. The effect in medium and high dose mulberry leaves polysaccharides is more significant.