Objective:To establish a method for determination of trachelogenin-4-O-β-gentiobioside

tracheloside

rhoifolin

trachelogenin and luteolin in Trachelospermum jasminoides. Method: The Alltima C18 column (4.6 mm×250 mm

5 μm) was used; the mobile phase consisting of 0.5%HAc-water. The flow rate was 1.0 mL·min-1; the detection wavelength set at 270 nm and content calculated with external reference method. Result: The five compounds were well separated. Calibration curves showed good linear relationship

RSD＜2.0%. The average recoveries of trachelogenin-4-O-β-gentiobioside

tracheloside

rhoifolin

trachelogenin and luteolin were 98.27%

97.30%

97.20%

96.89% and 96.48% respectively. Conclusion: The developed method is simple

accurate

and repeatable

which can be readily used as valid tool for the quality control of T. Jasminoides.