Objective: HPLC was employed to detect the tannin in salvianolic acid extracts for injection. Method: Gallic acid

catechins and ellagic acid in salvianolic acid extracts for injection were analyzed using HPLC method before and after the hydrolysis. The chromatographic condition for gallic acid was as follows:the chromatographic column was Venusil XBP C18 (4.6 mm×250 mm

5 μm) with the flow rate of 0.8 mL·min-1

mobile phase was phosphoric acid-water (0.05:100) and methanol

column temperature was kept at 30℃ and the detector was set at 271 nm. The chromatographic condition for catechins was as follows:the chromatographic column was Venusil XBP C18 (4.6 mm×250 mm

5 μm) at the gradient elution mode with the flow rate of 0.8 mL·min-1

mobile phase was phosphoric acid-water (0.05: 100) and methanol

column temperature was kept at 30℃ and the detector was set at 271 nm. The chromatographic condition for ellagic acid was as follows:the chromatographic column was Venusil XBP C18(4.6 mm×250 mm

5 μm) with the flow rate of 1.0 mL·min-1

mobile phase was phosphoric acid-water (0.2:100) and acetonitrile

column temperature was kept at 40℃ and the detector was set at 254 nm. Result: There was no gallic acid in salvianolic acid extracts before and after the hydrolysis. But catechins and ellagic acid might exist in salvianolic acid extracts after the hydrolysis. With the corresponding standard product in salvianolic acid extracts after the hydrolysis and detected again

it proved that no catechins and ellagic acid existed in salvianolic acid extracts after the hydrolysis. Conclusion: No tannin exists in salvianolic acid extracts after the treatment of polyamide resin.