Optimization of Extraction Technology for Total Saponins from  by Foam Separation

ZHANG Hai-bin; HE Yu-min; ZHANG Chang-cheng; GUAN Qiao-zhong; MAO Shuai; SUN Zhi-wei; YUAN Ding

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Optimization of Extraction Technology for Total Saponins from by Foam Separation

更新时间：2024-01-04

- Optimization of Extraction Technology for Total Saponins from by Foam Separation
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 1, Pages: 18-20(2013)
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- Published：2013
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ZHANG Hai-bin, HE Yu-min, ZHANG Chang-cheng, et al. Optimization of Extraction Technology for Total Saponins from by Foam Separation[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(1): 18-20.
DOI：

ZHANG Hai-bin, HE Yu-min, ZHANG Chang-cheng, et al. Optimization of Extraction Technology for Total Saponins from by Foam Separation[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(1): 18-20. DOI：

摘要

目的: 优选泡沫分离法提取竹节参总皂苷的工艺条件。方法: 以富集比、纯度比、回收率为指标

对气泡大小、原料液温度、电解质质量浓度及pH等影响泡沫分离的主要因素进行考察

采用单因素试验优化泡沫分离法提取竹节参皂苷的工艺条件。结果: 最佳提取工艺为气泡直径0.4~0.5 mm

温度65℃

pH 5.5

电解质NaCl摩尔浓度0.015 mol·L-1

总皂苷富集比2.1

纯度比2.6

回收率98.33%。结论: 泡沫分离法分离竹节参总皂苷方法稳定可靠

产品收率高

为天然竹节参皂苷的开发利用提供实验依据。

Abstract

Objective:To optimize extraction conditions of total saponins from Panax japonicus by foam separation technique. Method: Taking enrichment ratio

purity ratio and recovery as indexes

effect of pH

bubble size

temperature of mixture

electrolyte concentration and other main influencing factor on foam separation was investigated

single factor test was used to optimize extraction technology of total saponins from P. japonicus by foam separation. Result: Optimum extraction conditions were as followings:bubble diameter 0.4-0.5 mm

temperature 65℃

pH 5.6

the concentration of NaCl 0.015 mol·L-1

under these conditions

enrichment ratio of total saponins was 2.1

purity ratio was above 2.6 and recovery was 98.33%. Conclusion: Foam separation technique could be applied to separate total saponins from P. japonicus with stability

reliability and high yield

it provided a reference for development and utilization of natural total saponins in P. japonicus.

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