Inhibiting Effects of Flavonoids from  on Human Lung Carcinoma Cell Line A-549

ZHANG Cheng-jun; DENG Yan-ru; WANG Ying; MOU Jia-jia

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Inhibiting Effects of Flavonoids from on Human Lung Carcinoma Cell Line A-549

更新时间：2024-01-04

- Inhibiting Effects of Flavonoids from on Human Lung Carcinoma Cell Line A-549
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 22, Pages: 243-247(2012)
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- Published：2012
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ZHANG Cheng-jun, DENG Yan-ru, WANG Ying, et al. Inhibiting Effects of Flavonoids from on Human Lung Carcinoma Cell Line A-549[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(22): 243-247.
DOI：

ZHANG Cheng-jun, DENG Yan-ru, WANG Ying, et al. Inhibiting Effects of Flavonoids from on Human Lung Carcinoma Cell Line A-549[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(22): 243-247. DOI：

摘要

目的: 研究从沙漠绢蒿中得到的黄酮类化合物体外对肺癌A-549细胞增殖的影响。方法: 利用硅胶柱色谱等方法分离纯化6种黄酮类化合物

分别为5

4'-三羟基-6-甲氧基黄酮、5

7-二羟基-6

5'-四甲氧基黄酮、5

4'-三羟基-6

5'-三甲氧基黄酮、5

7-二羟基-6

4'-三甲氧基黄酮、槲皮万寿菊素-3

6-二甲醚-7-O-β-D-葡萄糖苷、木犀草素-7-O-β-D-葡萄糖苷-(3'-O-7″)-槲皮素-3″-甲醚;采用MTT法测定6种黄酮类化合物对肺癌A-549细胞增殖的抑制作用;采用流式细胞仪检测细胞周期及细胞凋亡。结果: 受试的6种黄酮类化合物中的5种化合物对肺癌A-549细胞增殖具有明显抑制作用

其抑制率与化合物浓度呈一定的相关性;流式细胞仪检测发现其中2种化合物(5

4'-三羟基-6-甲氧基黄酮和5

4'-三羟基-6

5'-三甲氧基黄酮)使细胞周期分别阻滞于G1和G2期

另1种化合物(5

7-二羟基-6

4'-三甲氧基黄酮)可通过诱导细胞凋亡及细胞周期阻滞抑制A-549细胞增殖。结论: 沙漠绢蒿黄酮对人肺癌细胞株A-549增殖具有明显抑制作用

使细胞发生周期阻滞或凋亡。

Abstract

Objective:To study the inhibitory effect of flavonoids from Seriphidium santolium on the proliferation of the lung carcinoma A549 cell line. Method: Six flavonoids were isolated by column chromatography on silica gel. They were identified as hispidulin

arteanoflavone

4'-trihydroxy-6

5'-trimethoxyflavone

eupatilin

quercetagetin-3

6-dimethyl ether-7-O-β-D-glucoside

luteolin-7-O-β-D-glucoside-(3'-O-7″)-quercetin-3″-methyl ether. MTT was performed to examine the proliferation of A-549 cells

and flow cytometry was used to determine the cell cycle distribution and cell apoptosis. Result: There was a dose-dependent inhibition of cell proliferation of A-549 by five compounds. The antiproliferative effect of compound Ⅰ(hispidulin) was attributed to its inducing cell cycle (G1) arrest

and compound Ⅲ(5

4'-trihydroxy-6

5'-trimethoxyflavone) attributed to its inducing cell cycle (G2) arrest. Compound Ⅳ (eupatilin) induced the apoptosis and inhibited the cell circle of A-549 cell. Conclusion: Flavonoids from S. santolium could exert an anti-lung cancer effect via induction of cell cycle arrest or cell apoptosis.

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