Objective: To determine optimum extraction technology by comparing traditional and modern extraction methods of Yinchenhao prescription. Method: Based on traditional decoction of Yinchenhao prescription

the content of index components were determined by UV spectrophotometry

with composite score of total chlorogenic acid

total iridoid glycosides

total anthraquinones and total polysaccharides as index

extraction technology of Yinchenhao prescription was optimized by single factor test and orthogonal test. Result: Optimum extraction process was as following:extracted 3 times with 12

8

6 times the amount of water

extraction time of 1

1

0.5 h

respectively;Then extracted 2 times with 6 times the amount of 80% ethanol

0.5 h per time. Conclusion: Different extraction methods had significant effects on the content of active ingredients in Yinchenhao prescription

two-step extraction method was better than other methods in extracting active ingredients from Yinchenhao prescription

and it was stable and feasible

it could provide experimental basis for reasonable application and further study of in Yinchenhao prescription.