A Study of Anti-adenovirus Effect of Andrographolide Sulfonated

PING Jing; WANG Si-yuan; XIE Ning; CHEN Xiao; WANG Xue-feng

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A Study of Anti-adenovirus Effect of Andrographolide Sulfonated

更新时间：2024-01-04

- A Study of Anti-adenovirus Effect of Andrographolide Sulfonated
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 21, Pages: 175-179(2012)
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- Published：2012
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PING Jing, WANG Si-yuan, XIE Ning, et al. A Study of Anti-adenovirus Effect of Andrographolide Sulfonated [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(21): 175-179.
DOI：

PING Jing, WANG Si-yuan, XIE Ning, et al. A Study of Anti-adenovirus Effect of Andrographolide Sulfonated [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(21): 175-179. DOI：

摘要

目的: 研究穿心莲内酯磺化物(ADS)体外抗腺病毒-3(AdV3)活性及细胞毒性。方法: 采用细胞病变法(CPE)、四甲基偶氮唑蓝比色法(MTT)检测ADS的最大无毒浓度(TC0)和半数中毒浓度(TC50)

以AdV3 100倍半数组织细胞感染量(100TCID50)攻击体外培养的人喉癌上皮细胞(Hep-2)造成AdV3感染模型

以利巴韦林注射液为对照药

以最小有效浓度(MEC)、半数有效浓度(EC50)及治疗指数(TI)为指标

测定了ADS 在6.10

12.21

24.41

48.23

97.66

195.31

390.63

871.25 mg ·L-1的体外抑制腺病毒活性。结果: ADS、利巴韦林注射液TC0分别为781.25

6.25 mg ·L-1; MEC分别为97.66

0.390 6 mg ·L-1;TI分别为8

16。结论: ADS97.66~781.25 mg ·L-1可有效抑制细胞内AdV3体外增殖

并呈明显的量效关系。

Abstract

Objective: To observe anti-adenovirus 3 (AdV3) effect and cytotoxicity of andrographolide sulfonated (ADS) in vitro. Method: The maximum nontoxic concentration (0% toxic concentration

TC0) and 50% toxic concentration (TC50) of ADS were analyzed by cytopathic effect (CPE) and methyl thiazolyl tetrazolium (MTT) colorimetric test. The AdV3 at 100TCID50(50% tissue cell infection dose) attacked Hep-2 cellin vitro culture to cause AdV3 infection model. The anti-adenovirus activity of ADS at 6.10

12.21

24.41

48.23

97.66

195.31

390.63

871.25 mg·L-1 in vitro were measured with ribavirin as control

and the minimum effective concentration (MEC)

50% effective concentration (EC50) and therapeutic index (TI) were used as the indicators. Result: ADS and ribavirin TC0 were 781.25 mg·L-1and 6.25 mg·L-1; MECs were 97.66 mg·L-1and 0.390 6 mg·L-1

TIs were 8 and 16

respectively. Conclusion: ADS 97.66-781.25 mg·L-1can effectively inhibit the intracellular proliferation of AdV3 in vitro

and showed a significant dose-effect relationship.

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