Objective:To determine and compare the main effective constituent (geniposide and crocin-1) content of Gardenia jasminoides in Chongqing Hou-shan and other domestic by ultra-performance liquid chromatography (UPLC). Method: The samples were separated at 30 ℃ on a Waters ACQUITY UPLC BEH C18 (2.1 mm×50 mm

1.7 μm) column eluted with methanol and water containing 0.2% phosphate acid as mobile phases in a linear gradient mode. Flow rate was set at 0.3 mL·min-1 and the detection wavelength were set at 238 nm and 440 nm. Result: The calibration curve of geniposide was linear within the range from 0.008 1 to 1.300 g·L-1 (r=0.999 9) and the average recovery was 99.6% (RSD 1.24%). The calibration curve of crocin-1 was linear within the range from 0.008 7 to 1.392 g·L-1 (r=0.999 9) and the average recovery was 101.0% (RSD 1.9%). Conclusion: The developed method was proved to be simple

reliable and useful by analysis of the samples from different areas

and the experimental result could provide scientific reference for quality evaluation and offer basis for further exploitation and utilization of G. jasminoides in Hou-Shan area

Chongqing Province.