Objective:To observe the effects of the separate application and time-dependent combination of Ziyin prescription(Liuwei Dihuang Pill

LWDHP) and Yiyang prescription (Jingui Shenqi Pill

JGSQP) on hypothalamus-pituitary-adrenal-thymus(HPAT) axis of kidney deficient aging model rats. Method: The rats were randomly divided into six groups. In addition to the normal group

other groups were injected 5%D-galactose solution 100 mg·kg-1 combined with glucocorticoid dexamethasone solution 0.5 mg·kg-1 to establish the model for six weeks

after establishing the model two weeks

each medication group was given LWDHP

JGSQP

LWDHP(morning use)combined with JGSQP (night use)

JGSQP (morning use) combined with LWDHP(night use)

1.16 g·kg-1 for four weeks

then the content of serum cortisol(COR)

plasma adrenocorticotropic hormone(ACTH)

corticotropin-releasing hormone(CRH)

and CD4+

CD8+were detected. Result: Compared with natural group

serum COR of kidney deficient aging model group significantly decreased(P<0.05)

plasma ACTH

CRH significantly increased(P<0.05)

the level of CD8+ significantly decreased(P<0.01)

CD4+/CD8+ significantly increased(P<0.01). Compared with kidney deficient aging model group

COR of Ziyin combined with Yiyang group(ZY group) significantly increased(P<0.01)

ACTH of Yiyang group

Yiyang combined with Ziyin group(YZ group) significantly decreased(P<0.05)

CRH of Ziyin group

ZY group

YZ group significantly decreased(P<0.05 or P<0.01)

the level of CD8+

CD4+/CD8+ have no significant differences

the level of CD4+ have no significant differences between each group. Conclusion: The effects of separate application and time-dependent combination of Ziyin prescription and Yiyang prescription on HPA axis of kidney deficient aging model rats are different

ZY prescription have the best effect

but these prescriptions have no effect on immunity.