Effect and Mechanism of -theanine on Improving Exercise Capacity in Rats

BAI Bao-feng

您当前的位置：

首页 >

文章列表页 >

Effect and Mechanism of -theanine on Improving Exercise Capacity in Rats

更新时间：2024-01-04

- Effect and Mechanism of -theanine on Improving Exercise Capacity in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 19, Pages: 201-204(2012)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2012
- 稿件说明：
移动端阅览
BAI Bao-feng. Effect and Mechanism of -theanine on Improving Exercise Capacity in Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(19): 201-204.
DOI：

BAI Bao-feng. Effect and Mechanism of -theanine on Improving Exercise Capacity in Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(19): 201-204. DOI：

摘要

目的:建立大鼠一次性力竭游泳模型

研究茶氨酸对大鼠纹状体递质性氨基酸及谷氨酸受体蛋白含量与酪氨酸磷酸化水平的影响

从而探讨其对提高运动能力的中枢机制。方法:雄性SD大鼠随机分为正常对照组、茶氨酸高、中、低剂量(400

200

100mg·kg-1)组

每组10只。各组大鼠适应性饲养1周后ig给药

正常对照组ig给予等体积生理盐水

共30d。第31天

比较各组大鼠游泳至力竭时间;断头取纹状体

高效液相色谱测定纹状体匀浆中谷氨酸(glutamic acid

Glu)、甘氨酸(glycine

Gly)、γ-氨基丁酸(γ-aminobutyric acid

GABA)与天冬氨酸(aspartic acid

ASP)含量

通过免疫印迹法与免疫沉淀法测定N-甲基-D-天冬氨酸受体亚基(N-methyl-D-aspartate receptor subunits 2A

NR2A)蛋白含量与酪氨酸磷酸化水平。结果:茶氨酸有效延长大鼠游泳时间(149.4±4.0)

(164.7±4.1)min

(P<0.01)

高剂量茶氨酸显著提高纹状体中Glu含量(10063.2±359.5)μmol·L-1;与对照组比较

高剂量茶氨酸提高NR2A蛋白相对含量(134.1±3.6)与酪氨酸磷酸化水平(123.2±4.4

对照组为100

显著降低GABA含量(1881.3±336.1)μmol·L-1

与正常对照组(2237.1±198.4)μmol·L-1比较

P<0.05。结论:茶氨酸能缓解急性运动导致的中枢神经系统兴奋性抑制

从而提高运动能力

机制可能与其提高兴奋性神经递质含量降低抑制性神经递质含量及提高谷氨酸受体活性有关。

Abstract

Objective:To investigate the influence of L-theanine on amino acids neurotransmitter and GluRs in rats by acute exhaustion swimming and mechanism of L-theanine improves exercise capacity. Method:SD male rats were randomly divided into high

middle and low dose (400

200

100 mg·kg-1

ig) groups and normal control group

(n=10

in each group). After one week

each group was started to give L-theanine for 30 days. The normal control group was given normal saline. On the day 31

rat exhaustive swimming time was measured. Meanwhile

Glu

Gly

GABA and Asp of corpus striatum in rats were determined by HPLC. Immunoprecipitation and immunoblotting were applied to analyze the level of NR2A and tyrosine phosphorylation. Result:Compared with the normal control group

swimming time in high and middle dose groups increased significantly . Level of Glu

NR2A

tyrosine phosphorylation showed obviously higher in high dose group than the normal control group·L-1

(134.1±3.6)

(123.2±4.4)

(P<0.05)]. High dose group also showed lower GABA(1 881.3±336.1)μmol·L-1 level than normal control group (2 237.1±198.4) μmol·L-1(P<0.05). Conclusion:L-theanine has the effect of improving exercise capacity in rats possibly through enhancing Glu

NMDAR2A

tyrosine phosphorylation level and inhibiting the synthesis or secretion of GABA in brain.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Cistanches Herba on the Swimming Ability and Oxidation Resistance of Mitochondrial in Rats

Effect of Morindae Officinalis Radix on the Exercise Capacity in Rats and Oxidation Resistance of Mitochondrial

Studies of Effects of Rhodiola Sachalinensis Complex Prescription on Exercise Capacity and Red Cell Immune Function in Rats

Related Author

LIN Qiang

CAO Jian-min

ZHOU Hai-tao

SHANG Xiao-xu

CHENG Ye

ZHOU Hai-tao

ZHU Chao

ZHOU Hai-tao1

Related Institution

Beijing Union University

Beijing Sport Universty

Beijing Xiaotangshan Hospital

Educational Research Center of Chaoyang District

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰