Objective: To establish a method of quality control of Swertia mussotii. Method: TLC method was applied in the qualitative identification of S. mussotii. The content of swertiamarin

mangiferin

swertianolin and oleanolic acid were determined by HPLC equipped with Diamonsil C18 (4.6 mm×250 mm

5 μm) column. The mobile phase of swertiamarin

mangiferin and swertianolin consisted of methanol-0.02% phosphoric acid

and detected at the wavelength of 254 nm. The mobile phase of oleanolic acid consisted of methanol-0.2% glacial acetic acid

and detected at the wavelength of 210 nm. The analysis was done at a flow rate of 1.0 mL·min-1. The column temperature was kept at 30℃. Result: The linearity of swertiamarin

mangiferin

swertianolin and oleanolic acid was in the range of 0.075-0.75 μg (r=0.999 9)

0.05-0.5 μg (r=0.999 7)

0.032-0.32 μg (r=0.999 3) and 0.116-11.6 μg (r=0.999 4)

respectively. The average recoveries were 98.91% (RSD 0.77%) for swertiamain

99.61% (RSD 1.38%) for mangiferin

100.35% (RSD 0.69%) for swertianolin and 99.26% (RSD 0.40%) for oleanolic acid respectively. Conclusion: This method is simple

accurate with good reproducibility. It is suitable for quality control of S. mussotii.