Objective: To study on preparation process of carboxylated multi-walled carbon nanotubes load hydroxycamptothecin(HCPT) and intestine absorption characteristics of HCPT-MWCNTs-COOH. Method: Optimum load method for HCPT-MWCNTs-COOH was selected by comparing adsorption capacity of oscillation method

milling method and ultrasonic method;FIR and UV spectroscopy were used to characterize

the content of it was determined by HPLC;Isolated everted intestine model was used to investigate absorption characteristics in different regions of rat intestine(duodenum

jejunum

ileum

colon)

the concentration of samples were determined by HPLC

and calculated absorption rate constant(Ka). Result: Ball milling method with frozen had the highest load(12.5 mg·g-1) for preparing HCPT-MWCNTs-COOH;FIR and UV spectroscopy changes showed carbon nanotubes had loaded HCPT.Intestine absorption test showed that Ka increased linearly along with increasing of HCPT concentration;Ka had no significant difference between different regions of intestines

Ka of the high concentration in duodenum

jejunum

ileum

colon were 150.9

92.7

148.9

183.0 ng·cm-2·h-1

respectively. Conclusion: Solubility of HCPT and intestine transmission rate in the load were increased and lactone ring was protected;The load in small intestine was passive diffusion absorption.