Objective: To establish a HPLC-ELSD method for determining contents of monosaccharide and oligosaccharide in Rehmannia glutinosa

and study on role of accessories in Wenzhi R. glutinosa with Jianchang band. Method: HPLC-ELSD was applied to determine the content of monosaccharide and oligosaccharide with prevail carbohydrate ES column(4.6 mm×250 mm

5 μm)

influence of accessories on Wenzhi R. glutinosa with Jianchang band was investigated. Result: The content of monosaccharide in Wenzhi R. glutinosa was higher than that of processing accessories negative contrast groups

but the content of oligosaccharide was just the opposite.The content of monosaccharide in Wenzhi R. glutinosa

Amomum villosum negative contrast group

A. villosum and Citrus reticulata negative contrast group

A. villosum

yellow rice wine and C. reticulata negative contrast group

yellow rice wine negative contrast group and C. reticulata negative contrast group were (23.46±1.07)%

(21.81±0.94)%

(15.83±1.01)%

(14.14±0.58)%

(21.91±0.76)%

(21.53±0.84)%

respectively;The content of oligosaccharide in them were (3.02±0.25)%

(3.29±0.17)%

(11.73±0.64)%

(14.33±0.83)%

(6.17±0.38)%

(3.77±0.19)%

respectively. Conclusion: This established determination method was simple

stable and accurate;It demonstrated that processing accessories could affect change of chemical component in processing of R. glutinosa

including A. villosum

yellow rice wine and C. reticulata.It could provid experimental basis to clarify processing mechanism of R. glutinosa.