Objective: To extract and separate the geniposide monomer rapidly

and study its analgesic and anti-inflammatory effects. Method: Macroporous resin was used to rich and isolate geniposide from Fruit Gardenia jiaminoides and medium pressure column chromatography system was used to purify geniposide monomer quickly. BALB/c mice were divided into 5 groups: normal control group (saline)

geniposide groups (50

100

200 mg·kg-1) and morphine group or aspirin group (5

100 mg·kg-1). Administrated by tail vein injection

hot plate test

acetic writhing test

xylene ear swelling test and the carrageenan paw edema test were used to evaluate the analgesic and anti-inflammatory activities of geniposide monomer in mice. Result: The content of geniposide was raised to 78.96% with macroporous resin

and 98.47% with medium-pressure column chromatography purification. Geniposide monomer could significantly reduce the writhing actions

which were induced by the intraperitoneal injection of acetic acid in mice

inhibit the xylene-induced ear swelling and carrageenan-induced paw swelling with a dose-dependent manner (P<0.05)

but it could not increase the hot plate pain threshold. Conclusion: Geniposide was separated and purified rapidly and successfully and it exhibited good analgesic and anti-inflammatory effects in mice.