Antioxidant Activities  of Polysaccharides from

WEI Xiu-juan; XIANG Fa-chun; CUI Ming-jun; XU Jian-rong

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Antioxidant Activities of Polysaccharides from

更新时间：2024-09-23

- Antioxidant Activities of Polysaccharides from
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 3, Pages: 197-200(2013)
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- Published：2013
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WEI Xiu-juan, XIANG Fa-chun, CUI Ming-jun, et al. Antioxidant Activities of Polysaccharides from [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 197-200.
DOI：

WEI Xiu-juan, XIANG Fa-chun, CUI Ming-jun, et al. Antioxidant Activities of Polysaccharides from [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 197-200. DOI：

摘要

目的: 研究不同醇沉浓度下铁苋菜多糖的体外抗氧化活性。方法: 用质量浓度为30%

45%

60%

75%

80%的无水乙醇分级沉淀的方法得到不同组分的铁苋菜多糖

分别采用高铁还原法、对二苯代苦味酰基(DPPH·)体系、光照核黄素法、硫代巴比妥酸法比较它们的总还原力(T-AOC)、对二苯代苦味酰基自由基(DPPH·)、超氧阴离子自由基(O2-·)、羟基自由基(·OH)的清除作用及对卵黄脂蛋白脂质过氧化物(LPO)的抑制效果。结果: 醇沉浓度为75%时对DPPH·的清除率达到了73.85%

其余各组分多糖都有良好的抗氧化效果

其中对O2-·和·OH的清除作用最为明显

清除率分别达到了92.47%和95.89%

几乎与抗坏血酸的清除作用相当

75%醇沉条件下的多糖对LPO也有较强的抑制作用。结论: 铁苋菜各组分多糖都有较强的抗氧化作用。

Abstract

Objective: To assess the antioxidant activities of polysaccharide from Acalypha australis L. by different ethanol concentration in vitro. Method: The different part of polysaccharide were precipitated by 30%

45%

60%

75%

80% ethanol. The scavenging rate of the precipitated polysaccharides on (DPPH·)free radical

superoxide anion radical(O2-·)

hydroxyl radicals(·OH) was investigated by ferric reducing method

DPPH·radical assay

O2-·radical assay and thiobarbituric acid method respectively. The total antioxidantive capacity (T-AOC) and the inhibition on lipid peroxide (LPO) of the precipitated polysaccharides were also been determined. Result: The scavenging activities on the DPPH·reached 73.85%. The results showed that T-AOC was enhanced with the increase of polysaccharide concentration. The antioxidantive especially the scavenging activities on the O2-·and·OH reached 92.47%

95.89% respectively at 5 g·L-1.Furthermore the scavenging effects were as much as that of vitamin C(VC). The inhibition of LPO with the polysaccharide precipitated by 75% ethanol was significantly higher than that of any by other ethanol concentrations. Conclusion: the precipitated polysaccharides from A. australis showed strong antioxidant activities.

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Related Author

WEI Xiu-juan

XIANG Fa-chun

CUI Ming-jun

XU Jian-rong

YU Xiang-dong1

CUI Jun2

ZHANG Hong-bin3

YANG Wen-ming1

Related Institution

School of Life Science and Engineering,Southwest Univerisity of Science and Technology

China University of Petroleum

Beijing Sport University

Beijing Union University

Department of Pharmacology,Guangxi Medical University

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