Effects of TanshinoneⅡ on Protein Expression of -type Calcium Channelα1c in Rat Atrial Fibrillation Model

LIU Wei-qin; MA Qing-hua; MOU Xia

您当前的位置：

首页 >

文章列表页 >

Effects of TanshinoneⅡ on Protein Expression of -type Calcium Channelα1c in Rat Atrial Fibrillation Model

更新时间：2024-01-04

- Effects of TanshinoneⅡ on Protein Expression of -type Calcium Channelα1c in Rat Atrial Fibrillation Model
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 4, Pages: 199-202(2013)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2013
- 稿件说明：
移动端阅览
LIU Wei-qin, MA Qing-hua, MOU Xia. Effects of TanshinoneⅡ on Protein Expression of -type Calcium Channelα1c in Rat Atrial Fibrillation Model[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(4): 199-202.
DOI：

LIU Wei-qin, MA Qing-hua, MOU Xia. Effects of TanshinoneⅡ on Protein Expression of -type Calcium Channelα1c in Rat Atrial Fibrillation Model[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(4): 199-202. DOI：

摘要

目的: 探讨丹参酮ⅡA(TanⅡA)对房颤(AF)大鼠心房肌细胞L型钙通道α1c亚单位(LTCCα1c或CaLα1c)mRNA表达的影响

揭示活血祛瘀中药预防和治疗房颤的作用机制。方法: 采用乙酞胆碱-氯化钙(Ach-CaCl2)药物经尾静脉注射法复制AF大鼠模型

随机分成3组:模型组、丹参酮治疗组、维拉帕米干预组

并设正常对照组。2周后

应用免疫组化方法和RT-PCR法检测心房肌细胞CaLα1c mRNA及蛋白表达变化。结果: 与正常对照组相比

模型组CaLα1c亚单位 mRNA表达水平降低

L型钙通道(LTCC)蛋白表达水平减低

两组比较具有差异性(P<0.05);与模型组比较

丹参酮治疗组、维拉帕米干预组均明显改善了房颤大鼠心房组织CaLα1c亚单位mRNA表达和蛋白表达水平减低状态(P<0.05)。结论: 丹参酮ⅡA能够改善房颤大鼠心房肌细胞重构过程的损伤程度

可能是其干预治疗房颤的靶点之一。

Abstract

Objective: To explore the possible mechanism and effect of tanshinoneⅡA on the protein expression of L-type calcium channelα1c in th rat atrial fibrillation model to reveal the mechanism of AF. Method: Rats were randomly divided into four groups

normal control group

model group

tanshinone ⅡA group and Verapamidronate group.The rat atrial fibrillation model was established by the tail vein injection of acetylcholine a calcium chloride (Ach-CaCl2) drug. The change of LTCCα1c expression was detected with immunohistochemistry after two weeks. Result: Compared with normal control group

in model group CaLα1c subunit mRNA expression level was reduced

L calcium channel (LTCC) protein expression level was reduced

there was a difference between two groups (P<0.05);compared with model group

in tanshinone treatment group and verapamil intervention group atrial fibrillation rats and atrial organization were significantly improved. CaLα1c subunit mRNA expression and protein expression level were reduced (P<0.05). Conclusion: TanshinoneⅡA could improve the decreasing status in AF rats and atrial muscle cell reconstruction process of the damage degree

which may be related with. CaLα1c subunit mRNA expression and protein expression.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Mechanism of Nardostachyos Radix et Rhizoma and Corydalis Rhizoma on Atrial Fibrillation Based on Network Pharmacology

Determination of Danshensu Dhpl,Tanshinone ⅡA,Dihydrotanshinone Ⅰ and Cryptotanshinone in Salvia miltiorrhiza Bge.by HPLC

Determination of Tanshinone Ⅱ in Radix Et Rhizoma Salviae Miltiorrhizae Microemulsion by HPLC

Determination of TanshinoneⅡ in Tiaoxuejiangzhi Pill by RP-HPLC

Related Author

Ting MAO

Jing-chun ZHANG

Bei LIU

Shan ZHANG

Yu-zhen AI

ZHENG Xiao-ke

DONG San-li

FENG Wei-sheng

Related Institution

Institute of Cardiovascular Diseases, Cardiovascular Diseases Center of Xiyuan Hospital, China Academy of Chinese Medical Sciences

School of Chinese Materia Medica,Beijing University of Chinese Medicine

China National Center for Biotechnology Development

1*),LIU Zi-dong~1

第四军医大学西京医院药剂科

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰