Effect of Guanxin Suhe Capsule on cTn-T and Myocardial Enzyme in Rat Acute Myocardial Infarction

HAO Di; LI Xu; KANG Li; WANG Zi; WANG Lei; LV Nan; CHONG Ying-ying; CHEN Wei-ping

您当前的位置：

首页 >

文章列表页 >

Effect of Guanxin Suhe Capsule on cTn-T and Myocardial Enzyme in Rat Acute Myocardial Infarction

更新时间：2024-01-04

- Effect of Guanxin Suhe Capsule on cTn-T and Myocardial Enzyme in Rat Acute Myocardial Infarction
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 23, Pages: 195-199(2012)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2012
- 稿件说明：
移动端阅览
HAO Di, LI Xu, KANG Li, et al. Effect of Guanxin Suhe Capsule on cTn-T and Myocardial Enzyme in Rat Acute Myocardial Infarction[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(23): 195-199.
DOI：

HAO Di, LI Xu, KANG Li, et al. Effect of Guanxin Suhe Capsule on cTn-T and Myocardial Enzyme in Rat Acute Myocardial Infarction[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(23): 195-199. DOI：

摘要

目的: 利用心肌梗死大鼠模型观察肌钙蛋白T(cTn-T)在心肌损伤中的动态变化并确定其达峰时间

进而观察冠心苏合胶囊在这时间点上对急性心肌梗死大鼠cTn-T 及心肌酶的影响。方法: 实验分为两个阶段

第一阶段(摸索cTn-T达峰时间):大鼠经结扎左冠状动脉前降支造成心肌梗死

分别在结扎后12

69 h检测cTn-T含量并确定其达峰时间。实验第二阶段(冠心苏合作用观察):取正常大鼠随机分为假手术对照组、模型对照组和卡托普利阳性药对照组

以及冠心苏合胶囊0.8

0.4 g·kg-1剂量组

均灌胃给药

每天1次

连续5 d

于第5天给药后1 h制备大鼠心肌梗死模型

存活大鼠给药治疗1次

在cTn-T达峰时间点测定各组大鼠cTn-T含量

测定各组大鼠天冬氨酸转氨酶(AST)、肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)等血清心肌酶

NBT心脏染色测定心肌梗死质量百分比

术前、结扎即刻、结扎31 h测心电图

取大鼠左心室用福尔马林固定进行组织病理学检查。结果: ①cTn-T达峰时间:通过12

69 h几个时间点cTn-T含量比较

大鼠左冠状动脉结扎31

48 h模型组与假手术组cTn-T含量有显著性差异(P<0.001)

确定结扎31 h为cTn-T出现高峰时间点;②在对冠心苏合胶囊的药效观察实验中

取31 h作为观察时间点

可见冠心苏合胶囊高剂量组大鼠血清中cTn-T和AST含量下降

与模型组比较差异有统计学意义(P<0.01

P<0.01)。③对心肌梗死质量百分比的影响

冠心苏合胶囊高剂量组大鼠心肌梗死质量比低于模型组

差异有统计学意义(P<0.05)。④对心电图的影响

在结扎即刻和结扎31 h冠心苏合高、低剂量组ST段抬高明显低于模型组;⑤心脏HE染色发现冠心苏合两剂量组心肌组织病理变化均减轻

其中

冠心苏合高剂量组减轻更为明显。结论: 在急性心肌梗死大鼠模型中

cTn-T表现出特异性和敏感性

并在31 h最接近达峰时间;冠心苏合胶囊能在该时间点降低心肌梗死大鼠cTn-T

AST含量

降低心肌梗死大鼠梗死心肌质量百分比。

Abstract

Objective: To evaluate the dynamic changing of troponin(cTn-T) and observe the treatment effect of Guanxin Suhe capsule on acute myocardial infarction(AMI) in rats and investigate its mechanism. Method: Rats were selected to establish acute myocardial infarction model. The rats were divided randomly into five groups and treated with pure water

high-dose Guanxin Suhe (0.8 g·kg-1)

low-dose Guanxin Suhe (0.4 g·kg-1)

captopril respectively for 5 days

then AMI model were established. cTn-T

aspartate aminotransferase(AST)

creatine kinase enzyme(CK)

MBisoenzyme of creatine kinase(CK-MB) was determined after ligating 31 h. Result: The area of myocardial infarction in the Guanxin Suhe capsule high dosage group was reduced

cTn-T and AST decreased

showing a statistical significance compared with the model group (P<0.05

P<0.01

P<0.01).The effect of high-dose and low-dose Guanxin Suhe capsule on ECG in acute myocardial infarction rats was observed

the heights of ST segment were reduced just after ligating 0 h and 31 h. Conclusion: Guanxin Suhe capsule has significant effect on myocardial infarction

it treats myocardial infarction through regulating cTn-T and AST.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Linggui Zhugantang on Ventricular Remodeling After Myocardial Infarction and RhoA/ROCK Signaling Pathway

Ginsenoside Rg₂ Protects Heart After Acute Myocardial Infarction by Regulating PI3K/Akt/mTOR Signaling Pathway

Yangxin Dawayimicol Honey Ointment Improves Cardiac Function in Rats by Regulating VDAC1/NLRP3/Bax/Bcl-2 Pathway

Regulation of Signaling Pathways Related to Myocardial Infarction by Traditional Chinese Medicine： A Review

Effect of Qingxin Jieyu Granules Regulating Mitophagy on Ventricular Remodeling After Myocardial Infarction of C57B/L6 Mice

Related Author

REN Han

ZHAO Wanzhu

WANG Shushu

CAI Rui

ZHANG Yuanhong

HUANG Shengyi

HUANG Jinling

ZHANG Xixian

Related Institution

Anhui Province Key Laboratory of Chinese Medicinal Formula

School of Chinese Medicine，Anhui University of Chinese Medicine

School of Integrated Chinese Medicine and Western Medicine，Anhui University of Chinese Medicine

Henan University of Chinese Medicine

School of Basic Medicine and Forensic Medicine，Henan University of Science and Technology

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰