Objective:To study the compatibility of San'ao decoction. Method: Based on orthogonal design

mice was divided into 16 groups

16 in each group according to L16(43) table. Subcutaneous sensitization was undertaken on day 1 and 8

5% OVA atomization was applied to stimulate sensitization from day 15 to 28

before which San'ao Decoction with the corresponding compatibility proportion was given. Airway reactivity

the number of eosinophils (EOS) in blood

ESO in BALF

and white blood cells

pathological change of the lung and trachea were detected on the 29th day. To confirm the experiments

48 mice were divided into 3 groups: blank group

model group and optimum compatibility group with 16 in each. The above mentioned methods and indicators were applied and detected. Result: Based on synthetic sores

the optimum of San'ao decoction was as follows: 6 g Ephedrae Herba; 9 g Armeniacae Semen Amarum; and 3 g Radix et Rhizoma Glycyrrhizae. The sequence of priority was like: Ephedrae Herba>Armeniacae Semen Amarum>Radix et Rhizoma Glycyrrhizae. The confirmed experiments showed significant difference(P＜0.01) in airway reactivity

the number of EOS in blood

ESO in BALF

and white blood cells

pathological change of the lung and trachea compared with model(A1B1C1). Conclusion: The optimum compatibility proportion of San'ao decoction is similar to that used in clinical practice; and their sequence of priority confirms the compatibility principle of San'ao decoction.