Objective:To screen the antitumor extract of compound Fomes fomentarius

detect its chemical composition preliminarily and evaluate its antitumor activity in vitro. Method: Distilled water or ethanol was used for extraction of compound F. fomentarius. The MTT assay was used to measure the inhibition effect in vitro of distilled water extract of compound F. fomentarius (WECF) and ethanol extract of compound F. fomentarius (EECF). The system pre-test method was used to detect the chemical composition preliminarily. Apoptosis of A549 was tested by flow cytometry. Result: The 50% inhibition concentration (IC50) of WECF to A549

Hela and QGY were (0.28±0.02)

(0.40±0.06)

(0.28±0.05) g·L-1 respectively

and to EECF

the IC50 were (0.50±0.04)

(0.50±0.03)

(0.60±0.06) g·L-1 respectively. There were organic acid

amino acid

peptides and proteins

sugar

polysaccharide and nucleoside

saponins

lactone

coumarone and nucleoside

plant sterol and terpene composition

volatile oil and grease in WECF. The polysaccharide content of WECF was (16.1±0.5)%. The IC50 of WECF to A549

Hela

QGY

DU145

MDA-MB-435S and SGC-7901 were 0.29

0.45

0.32

0.32

0.83

0.23 g·L-1. 22.0% of totle cells apoptosis after A549 treating with WECF at 1 g·L-1 for 24 h. Conclusion: WECF showed higher antitumor activity in vitro compared with EECF and has significant inhibitory effect to a variety of tumor cells

further investigation revealed that WECF exerted an antitumor activity via apoptosis-induced cell death and maybe developed as a new antitumor medicine.