Effect of Tongxinluo on H9c2 Cardiomyocytes Hypertrophy and Expression of Connexin43 mRNA Induced by Isoproterenol
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Effect of Tongxinluo on H9c2 Cardiomyocytes Hypertrophy and Expression of Connexin43 mRNA Induced by Isoproterenol
Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 23, Pages: 191-194(2012)
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Published:2012
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YANG Jun, DING Sai-liang, DENG Biao, et al. Effect of Tongxinluo on H9c2 Cardiomyocytes Hypertrophy and Expression of Connexin43 mRNA Induced by Isoproterenol[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(23): 191-194.
DOI:
YANG Jun, DING Sai-liang, DENG Biao, et al. Effect of Tongxinluo on H9c2 Cardiomyocytes Hypertrophy and Expression of Connexin43 mRNA Induced by Isoproterenol[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(23): 191-194.DOI:
Effect of Tongxinluo on H9c2 Cardiomyocytes Hypertrophy and Expression of Connexin43 mRNA Induced by Isoproterenol
Objective: To investigate the effects of Tongxinluo (TXL) on hypertrophy of H9c2 cardiomyocytes and connexin 43 mRNA expression induced by isoproterenol (Iso). Method: Cultured H9c2 cardiomyocytes were randomly divided into four groups: control group
TXL group
Iso group
and Iso+TXL group. After 72 hours of culture
cell size was determined by phase contrast microscope
the protein concentrations were measured by BCA method and the expression of Cx43 mRNA were measured by reverse transcription polymerase chain reaction (RT-PCR). Result: Cell size of H9c2 cardiomyocytes of the Iso group (P<0.05) was increased more prominently than the control group. Compared with Iso group
Tongxinluo attenuated the increase of cardiomyocytes size (P<0.05). H9c2 cardiomyocytes protein concentrations in the Iso group (P<0.05) was increased more significantly than the control group. Although Tongxinluo didn't affect the normal H9c2 cardiomyocytes protein concentration
it inhibited the increase of protein concentrations induced by Iso (P<0.05).Iso decreased Cx43 mRNA expression of H9c2 cardiomyocytes more evidently than that of the control group (P<0.05)
which was inhibited by Tongxinluo (P<0.05). Conclusion: Tongxinluo can prevent the hypertrophy of H9c2 cardiomyocytes and down-regulate of Cx43 mRNA expression induced by Iso.
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Related Institution
Beijing University of Chinese Medicine
Beijing Key Laboratory of Chinese Materia Pharmacology,Institute of Basic Medical Sciences, Xiyuan Hospital,China Academy of Chinese Medical Sciences
Natural History Museum of China
School of Graduate,Hebei University of Chinese Medicine
Key Disciplines of Cardio-cerebral Vessel Collateral Disease, National Administration of Traditional Chinese Medicine(TCM), State Key Laboratory of Collateral Disease Research and Innovative of TCM, Hebei Yiling Pharmaceutical Research Institute