Objective:To find the protective role of compound denshen dripping pills(CDDP) on acute myocardial ischemia induced by isoproterenol(ISO) in rat

and it in apoptosis-related mechanism. Method: Rats were divided into: normal group

model group

compound Danshen Dripping Pill group (referred to as the compound group)

the tim Seoul heart group. Rats of each group were in advance administrated for one week

the compound group dose 80 mg·kg-1·d-1

Tim dongle group dose 16 mg·kg-1·d-1

the normal group and model group were given normal saline. Subcutaneous injection of large doses of ISO 20 mg·kg-1·d-1was used to induce model

for two days. The hemodynamics by multichannel physiologic recorder was recorded with PE50 catheter into left ventricle through right arteria carotis. Content changes of superoxide dismutase(SOD)

malondialdehyde(MDA)

troponin I(TnI) in serum were tested. The myocardial tissue paraffin section was obtained for HE staining to observe pathological changes of myocardial tissue. Expression changes of apoptosis-related proteins apoptosis signal regulating kinase 1(ASK-1)and caspase-9 were detected using Western blot. Result: In model group

hemodynamics of systolic blood pressure(SBP)

diastolic blood pressure(DBP)

and mean arterial blood pressure(MBP)

heart rate(HR)

left ventricular systolic pressure(LVSP) and left ventricular end diastolic blood pressure(LVEDP)

left ventricular pressure the greatest change in systolic and diastolic rate(± dp/dtmax) were significantly lower compared with the normal group (P<0.05). Compared with model group

in the compound group

hemodynamic parameters

in addition to LVEDP and +dp/dtmax

were statistically significant(P<0.05). In model group

serum SOD levels compared with normal group was significantly increased (P<0.05); the compound group SOD levels compared with the model group was significantly elevated (P<0.05). Serum MDA and TnI in model group were significantly increased compared with the normal group (P<0.05)

the compound group compared with the model group

MDA and TnI were decreased statistically (P<0.05). Western blot results showed that in the model group

the ASK-1

caspase-9 protein expression was significantly increased compared with the normal group (P<0.05)

the ASK-1 and caspase-9 protein expression of the compound group were significant decreased compared with the model group(P<0.05). Conclusion: CDDP has a protective role on acute myocardial ischemia in ISO-induced rat model

and the target may be involved in myocardial apoptosis associated signal conditioning.