Effects of Buyang Huanwu Decoction Containing Serum on the Morphology and Viability of PC12 Cells During Oxygen-glucose Deprivation

LIU Jun-e; ZHANG Ji-ping; YAO Hui; FANG Yong-qi; LIANG Yi; WEN Feng-ni

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Effects of Buyang Huanwu Decoction Containing Serum on the Morphology and Viability of PC12 Cells During Oxygen-glucose Deprivation

更新时间：2024-01-04

- Effects of Buyang Huanwu Decoction Containing Serum on the Morphology and Viability of PC12 Cells During Oxygen-glucose Deprivation
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 19, Pages: 186-190(2012)
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- Published：2012
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LIU Jun-e, ZHANG Ji-ping, YAO Hui, et al. Effects of Buyang Huanwu Decoction Containing Serum on the Morphology and Viability of PC12 Cells During Oxygen-glucose Deprivation[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(19): 186-190.
DOI：

LIU Jun-e, ZHANG Ji-ping, YAO Hui, et al. Effects of Buyang Huanwu Decoction Containing Serum on the Morphology and Viability of PC12 Cells During Oxygen-glucose Deprivation[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(19): 186-190. DOI：

摘要

目的:探讨补阳还五汤含药血清对缺氧缺糖(oxygen-glucose deprivation

OGD)损伤PC12细胞形态和活力的影响。方法:采用中药血清药理学方法制备补阳还五汤含药血清

以5%

10%

20%的含药血清作用于OGD损伤PC12细胞

于倒置显微镜下观察细胞形态

用四甲基偶氮唑盐法检测细胞活力。结果:PC12细胞OGD损伤后形态发生改变、细胞活力降低;5%

10%补阳还五汤含药血清作用于OGD损伤PC12细胞12

24h

均可减轻细胞形态损伤、提高细胞活力(P<0.01);而20%补阳还五汤含药血清随作用时间的延长对OGD损伤PC12细胞的形态和活力具有双向调节作用。结论:一定浓度的补阳还五汤含药血清对OGD损伤PC12细胞的形态和活力具有保护作用。

Abstract

Objective:To investigate effects of Buyang Huanwu decoction containing serum on the morphology and viability of PC12 cells during oxygen-glucose deprivation. Method:Buyang Huanwu decoction containing serum was obtained by the seropharmacological method. PC12 cells were treated with different concentrations (5%

10%

20%) of the serum during oxygen-glucose deprivation. The cell morphology was observed with inverted microscopy

and the cell viability was examined by methyl thiazolyl tetrazolium assay. Result:The cell morphology was changed and the cell viability was decreased during oxygen-glucose deprivation

and the injured degree of cell morphology was relieved and the cell viability was increased in PC12 cells which treated with concentrations of 5%

10% Buyang Huanwu decoction medicated serum for 12

24 h(P<0.01). However

20% Buyang Huanwu decoction containing serum had the bidirectional regulation effects on the morphology and viability of PC12 cells during oxygen-glucose deprivation. Conclusion:The certain concentration of Buyang Huanwu decoction congtaining serum has protective effects on the morphology and viability of PC12 cells during oxygen-glucose deprivation.

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Related Institution

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