Effects of Xinglou Chengqi Decoction and Buyang Huanwu Decoction on Fas/Fasl and Caspase-3 Pathway of Apoptosis in Rats with Cerebral Ischemia

LIU Jing-xia; LI Jian-sheng; YU Wei; HEI Chang-chun; LIU Hui-xian; REN Fei-fei

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Effects of Xinglou Chengqi Decoction and Buyang Huanwu Decoction on Fas/Fasl and Caspase-3 Pathway of Apoptosis in Rats with Cerebral Ischemia

更新时间：2024-01-04

- Effects of Xinglou Chengqi Decoction and Buyang Huanwu Decoction on Fas/Fasl and Caspase-3 Pathway of Apoptosis in Rats with Cerebral Ischemia
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 23, Pages: 187-191(2012)
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- Published：2012
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LIU Jing-xia, LI Jian-sheng, YU Wei, et al. Effects of Xinglou Chengqi Decoction and Buyang Huanwu Decoction on Fas/Fasl and Caspase-3 Pathway of Apoptosis in Rats with Cerebral Ischemia[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(23): 187-191.
DOI：

LIU Jing-xia, LI Jian-sheng, YU Wei, et al. Effects of Xinglou Chengqi Decoction and Buyang Huanwu Decoction on Fas/Fasl and Caspase-3 Pathway of Apoptosis in Rats with Cerebral Ischemia[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(23): 187-191. DOI：

摘要

目的: 观察星蒌承气汤和补阳还五汤对脑缺血神经细胞凋亡Fas/Fasl和Caspase-3的影响

以明确其阻抑神经细胞凋亡的机制。方法: 大鼠随机分为假手术组、模型组、尼莫地平组、星蒌承气汤和补阳还五汤组;线栓法制备大脑中动脉阻塞模型;星蒌承气汤(5.0 g·kg-1)、补阳还五汤(13.0 g·kg-1)、尼莫地平(10.8 mg·kg-1)组大鼠分别于造模前4 d灌胃

造模后每日1次;缺血后1

7 d取大鼠脑组织

免疫组织化学法检测Fas

Fasl

Caspase-3表达。结果: 假手术组大鼠可见少许Fas(16.60±1.36)

Fasl(19.40±1.72)和Caspase-3(16.35±1.63)表达;大鼠缺血后1

7 d的Fas(45.83±1.44

36.25±1.60

31.37±2.27)

Fasl(44.27±2.25

37.68±2.01

34.15±1.55)和Caspase-3(37.18±2.78

29.50±2.07

25.26±3.04)表达均增强(P<0.01);与模型组比较

各用药组Fas

Fasl表达减弱

尼莫地平组缺血后1 d

星蒌承气汤和补阳还五汤各组Caspase-3表达减弱;各星蒌承气汤和补阳还五汤组7 d的Fas

Fasl表达、星蒌承气汤组3 d的Caspase-3表达均较尼莫地平组减弱;星蒌承气汤组缺血后1 d的Fas和Fasl

3 d的Fas和Caspase-3表达均较补阳还五汤组减弱。结论: 脑缺血可引起细胞凋亡Fas/Fasl调控的表达上调

星蒌承气汤和补阳还五汤可下调Fas/Fasl及Caspase-3表达

星蒌承气汤阻抑Fas和Fasl及Caspase-3的作用较补阳还五汤早而显著

以缺血后1 d的调控作用最为明显。

Abstract

Objective: To observe the effects and mechanism of Xinglou Chengqi decocotion (XLCQD)and Buyang Huanwu decoction (BYHWD) on Fas/Fasl and Caspase-3 of regulating genes of neurons apoptosis caused by cerebral ischemia. Method: Rats were randomly divided into sham

model

Nimodipine

BYHWD and XLCQD groups. Focal cerebral ischemia models were established by middle cerebral artery occlusion with nylon thread. Rats were given with XLCQD(5.0 g·kg-1)

BYHWD(13.0 g·kg-1)and nimodipine(10.8 mg·kg-1)by ig before the model preparation

once a day after the operation. At 1

7 d after operation

neurons apoptosis

expressions of Fas

Fasl and Caspase-3 were determined using the method of immunohistochemistry. Result: A few expressions of Fas(16.60±1.36)

Fasl (19.40±1.72)and Caspase-3 (16.35±1.63)could be observed in rat ’s brain of sham group. In each model group

expressions of Fas(45.83±1.44

36.25±1.60

31.37±2.27)

Fasl(44.27±2.25

37.68±2.01

34.15±1.55)

Caspase-3(37.18±2.78

29.50±2.07

25.26±3.04)all increased(P<0.01). Fas and Fasl expression in each administrated group all decreased

and the expression of Caspase-3 in Nimodipine 1 d group

each XLCQD and BYHWD group was higher. Fas and Fasl expressions in each XLCQD group and 7 d BYHWD group and Caspase-3 in 3 d XLCQD group all decreased than that in Nimodipine groups. The expressions of Fas in 1 d and 3 d

Fasl in 1d

Caspase-3 in 3 d XLCQT groups all decreased than that in BYHWD groups. Conclusion: It was shown that Fas/Fasl up-regulation of apoptosis could be caused by cerebral ischemia

and XLCQD and BYHWD could all inhibit the level of Fas

Fasl and Caspase-3.The role of XLCQD was more earlier and significant in down-regulating the expressions of Fas

Fasl and Caspase-3

especially at 1d after cerebral ischemia.

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