Chinese Journal of Experimental Traditional Medical FormulaeVol. 19, Issue 2, Pages: 90-93(2013)
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Published:2013
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JIN Feng, REN Yu-zhen, CHEN Yan-lin, et al. Study on HPLC Fingerprint of Alkaloids in [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(2): 90-93.
DOI:
JIN Feng, REN Yu-zhen, CHEN Yan-lin, et al. Study on HPLC Fingerprint of Alkaloids in [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(2): 90-93.DOI:
目的: 建立巴豆生物碱部位的高效液相指纹图谱。 方法: 色谱柱Waters Symmetry C18(4.6 mm×250 mm
5 μm)
甲醇-0.2%乙酸水流动相系统梯度洗脱
流速0.8 mL·min-1
检测波长292 nm
温度28℃。 结果: 对10批巴豆药材进行了测定
建立了巴豆生物碱部位的指纹图谱
共有13个共有峰
指认了两种主要生物碱巴豆苷和木兰花碱。 结论: 该方法可为巴豆的研究和质量控制提供依据。
Abstract
Objective: To study chromatography fingerprints of the alkaloids in Croton tiglium by HPLC. Method: Waters Symmetry C18 column (4.6 mm×250 mm
5 μm) was used with methanol-0.2% acetic acid of water gradient elution
at flow-rate of 0.8 mL· min-1
detection wavelength at 292 nm; column temperature was at 25℃. Result: HPLC was used to establish fingerprint chromatograph of methanol extraction from thirteen batches of C. tiglium
which had been harvested from the same area at the same time. Crontonoside and magnoflorine in the fingerprint were identified. The result showed that peaks were common. Conclusion: The method could be used for the research and the quality control of C. tiglium.