Content Determination of Notoginsenoside R, Ginsenoside Rg and Ginsenoside Rb in Mingmu Granule by HPLC

YANG Meng-yu; WANG Jian; SUN Yi-kun

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Content Determination of Notoginsenoside R, Ginsenoside Rg and Ginsenoside Rb in Mingmu Granule by HPLC

更新时间：2024-01-04

- Content Determination of Notoginsenoside R, Ginsenoside Rg and Ginsenoside Rb in Mingmu Granule by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 23, Pages: 83-85(2012)
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- Published：2012
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YANG Meng-yu, WANG Jian, SUN Yi-kun. Content Determination of Notoginsenoside R, Ginsenoside Rg and Ginsenoside Rb in Mingmu Granule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(23): 83-85.
DOI：

YANG Meng-yu, WANG Jian, SUN Yi-kun. Content Determination of Notoginsenoside R, Ginsenoside Rg and Ginsenoside Rb in Mingmu Granule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(23): 83-85. DOI：

摘要

目的: 建立明目颗粒中三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1的含量测定方法。方法: 采用HPLC

Dikma Diamonsil(钻石)C18色谱柱(4.6 mm ×150 mm

5 μm)

流动相乙腈(A)-水(B)进行梯度洗脱

流速1 mL·min-1

检测波长203 nm

柱温25 ℃

进样量10 μL。结果: 三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1的线性范围分别为0.352~2.112 μg(r=0.999 3)

0.914~5.484 μg(r=0.999 2)

0.910~5.460 μg(r=0.999 1);平均加样回收率分别为99.4%

102.72%

101.89%

RSD分别为2.56%

2.16%

2.16%。结论: 本试验建立的测定方法简便、重复性好、精密度高

可用于该制剂的质量控制。

Abstract

Objective: To establish a method for determination of notoginsenoside R1

ginsenoside Rg1

ginsenoside Rb1 in Mingmu granule. Method: HPLC was used

Dikma Diamonsil C18 column (4.6 mm×150 mm

5μm)

mobile phase of acetonitrile and water with gradient elution

flow rate was 1.0 mL·min-1 and detection wavelength was at 203 nm

column temperature was 25 ℃

injection volume 10 μL. Result: Linear ranges of notoginsenoside R1

ginsenoside Rg1 and ginsenoside Rb1 were 0.352-2.112(r=0.999 3)

0.914-5.484(r=0.999 2)

0.910-5.460 μg (r=0.999 1)

respectively.Their average recoveries were 99.4% (RSD 2.56%)

102.72% (RSD 2.16%) and 101.89% (RSD 2.16%)

respectively. Conclusion: This established determination method was simple

accurate and reproducible

thus it could be used as quality control of Mingmu granule.

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