Inhibitory Effect of Serum Containing  on Hepatitis B Virus

CHEN Xiao-bai; WANG Xiao-ping; ZHAO Shi-hua

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Inhibitory Effect of Serum Containing on Hepatitis B Virus

更新时间：2024-01-04

- Inhibitory Effect of Serum Containing on Hepatitis B Virus
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 22, Pages: 218-221(2012)
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- Published：2012
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CHEN Xiao-bai, WANG Xiao-ping, ZHAO Shi-hua. Inhibitory Effect of Serum Containing on Hepatitis B Virus [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(22): 218-221.
DOI：

CHEN Xiao-bai, WANG Xiao-ping, ZHAO Shi-hua. Inhibitory Effect of Serum Containing on Hepatitis B Virus [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(22): 218-221. DOI：

摘要

目的: 观察毛鸡骨草(Abrus mollis)含药血清体外抗乙型肝炎病毒(HBV)的作用。方法: 采取中药血清药理实验方法

以HepG2.2.15细胞为研究模型

将毛鸡骨草含药血清加入HepG2.2.15细胞培养液中培养

分别在72 h和144 h收集细胞培养上清液

采用酶联免疫吸附实验法(ELISA法)和荧光定量PCR(FQ-PCR)法检测毛鸡骨草含药血清对HepG2.2.15细胞株表达的乙型肝炎表面抗原(HBsAg)和HBV DNA复制的影响。结果: 毛鸡骨草含药血清对HepG2.2.15细胞上清液中HBsAg的表达和HBV DNA复制都有不同程度的抑制

以给药剂量为30 g·kg-1·d-1的毛鸡骨草含药血清组作用144 h对HBsAg的抑制率和对HBV DNA复制的抑制率最明显

抑制率分别为34.4%和41.7%。结论: 毛鸡骨草在体外有一定的抗HBV作用。

Abstract

Objective:To observe the inhibitive effect of the serum containing Abrus mollis on hepatitis B virus(HBV). Method: HepG2.2.15 cells were cultured in medium containing the serum with A. mollis for 72 h and 144 h. The Effect of the serum containing A. mollis on HBsAg and HBV DNA reproduction in culture media of the HepG2.2.15 cells were determined by ELISA and fluorescence quantitative pdymerase chain reaction(FQ-PCR). Result: The serum containing A. mollis had a inhibiting effect on the HBsAg and HBV DNA reproduction with maximum inhibition ratio adding up to 34.4% and 41.7% respectively in which the affective time is 144 h and the density is 30 g·kg-1·d-1. Conclusion: A. mollis can significantly inhibit HBV in vitro.

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