Content Determination of Chlorogenic Acid, 3,4-- Dicaffeoylquinic Acid,3,5--Dicaffeoylquinic Acid in Chrysanthemi Indici Flos of Different Areas

WANG Li-guo; LIU Wei; WU Ming-xia

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Content Determination of Chlorogenic Acid, 3,4-- Dicaffeoylquinic Acid,3,5--Dicaffeoylquinic Acid in Chrysanthemi Indici Flos of Different Areas

更新时间：2024-01-04

- Content Determination of Chlorogenic Acid, 3,4-- Dicaffeoylquinic Acid,3,5--Dicaffeoylquinic Acid in Chrysanthemi Indici Flos of Different Areas
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 20, Pages: 75-78(2012)
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- Published：2012
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WANG Li-guo, LIU Wei, WU Ming-xia. Content Determination of Chlorogenic Acid, 3,4-- Dicaffeoylquinic Acid,3,5--Dicaffeoylquinic Acid in Chrysanthemi Indici Flos of Different Areas[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(20): 75-78.
DOI：

WANG Li-guo, LIU Wei, WU Ming-xia. Content Determination of Chlorogenic Acid, 3,4-- Dicaffeoylquinic Acid,3,5--Dicaffeoylquinic Acid in Chrysanthemi Indici Flos of Different Areas[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(20): 75-78. DOI：

摘要

目的: 建立不同产地野菊花中绿原酸、3

4-二咖啡酰奎尼酸、3

5-二咖啡酰奎尼酸的含量测定。方法: 采用HPLC方法

色谱柱为Diamonsil (4.6 mm ×250 mm

5 μm)

甲醇-0.1%磷酸溶液做流动相进行梯度洗脱

检测波长326 nm

柱温25 ℃

流速0.8 mL·min-1。结果: 绿原酸、3

4-二咖啡酰奎尼酸和3

5-二咖啡酰奎尼酸的线性范围分别为1.00~20.00

4.64~92.80

2.233~44.66 mg·L-1;加样回收率分别为98.97%

99.14%;99.39%

RSD分别为 1.81%

1.66%

1.36% (n=6)。结论: 方法操作简便

分析速度快

结果准确

重复性好

可用于野菊花中绿原酸、3

4-二咖啡酰奎尼酸、3

5-二咖啡酰奎尼酸的含量测定。

Abstract

Objective:To determine the content of chlorogenic acid

4-O-dicaffeoylquinic acid

5-O-dicaffeoylquinic acid in Chrysanthemi Indici Flos of different areas. Method: HPLC was used. The chromatographic separation was performed on a Diamonsil C18(4.6 mm × 250 mm

5 μm) column eluted with gradient mobile phase of methanol-0.1%H3PO4

the detection wavelength was set at 326 nm and column temperature was set at 25 ℃. Result: Nice linear relation between the area and content when the amount was within 1.00-20.00

4.64-92.80

2.233-44.66 mg·L-1.The recoveries were 98.97%

99.14% and 99.39% respectively

RSD was 1.81%

1.66% and 1.36% (n=6) respectively. Conclusion: The method is accurate

reproducible and suitable to determine these active components in Chrysanthemi Indici Flos. It can be used for quality control of Chrysanthemi Indici Flos.

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