Objective:To optimize extraction and purification technology of Buxin Ruanmai granule. Method: With transfer rate of salidroside and yield of extract as comprehensive evaluation indexes

the amount of water

extraction time and extraction times were chosen as investigated factors

orthogonal design was used to optimize water extraction process of Rhodiola crenulata and four other Chinese herbal pieces from Buxin Ruanmai granule;With transfer rate of total flavonol glycoside as index

ethanol extraction technology of Ginkgo biloba piece was investigated by single factor test;With transfer rate and cleaning rate of salidroside as indexes

alcohol precipitation technology of water extraction liquid from Buxin Ruanmai granule was optimized by single factor test. Result: Optimized water extraction technology of R. crenulata and four other Chinese herbal pieces was:extracted 3 times with 8 times the amount of water

0.5 h each time;Optimized alcohol extraction technology was:extracted 2 times with 10 times the amount of 70% ethanol

1 h per time;Optimal alcohol precipitation conditions were as follows:water extraction liquid concentrated to its relative density of 1.10-1.15

slowly added 95% ethanol until the concentration of ethanol of 70%

stirring at the same time

precipitated 24 h in cold. Conclusion: This optimized technology was simple

it could provide experimental basis for large-scale production of Buxin Ruanmai granule.