Objective: To develop an HPLC method for determination of 6 indicative components(gallic acid

hydroxyl-paeoniflorin

paeoniflorin

1

2

3

4

6-pentagalloylglucose

paeonol

benzoyl-paeoniflorin) in Moutan Cortex. Method: Phenomsil(4.6 mm×250 mm

5 μm) was adopted;the mobile phase was acetonitrile(A)-0.1% phosphoric acid solution(B) with gradient elution at a flow rate of 1.0 mL·min-1

and the detection wavelength was 267 nm(0-20 min

gallic acid)

258 nm(20-30 min

hydroxyl-paeoniflorin)

230 nm(30-50 min

paeoniflorin) 274 nm(50-80 min

1

2

3

4

6-pentagalloylglucose

paeonol)

230 nm(80-90 min

benzoyl-paeoniflorin). Result: The content of 6 indicative components in Moutan Cortex was stable. The method had a good linearity in the ranges of 0.109 4-1.094 μg(r=0.999 5)for gallic acid

0.034 86-0.348 6 μg(r=0.999 5) for hydroxyl-paeoniflorin

0.212 4-2.124 μg(r=0.999 1) for paeoniflorin

0.214 5-2.145 μg(r=0.999 6) for 1

2

3

4

6-pentagalloylglucose

0.323 5-3.235 μg(r=0.999 4) for paeonol

0.075 84-0.758 4 μg(r=0.999 3) for benzoyl-paeoniflorin. The average recoveris(n=6) were 98.7%

98.8%

99.7%

98.3%

98.9%

99.2%

respectively. Conclusion: This method is dependable

simple and practical

and may be use to control quality of Moutan Cortex.