Simultaneous Determination of Six Components in Huangbai by HPLC

LIAN Lian; WAN Guo-sheng; JIA Li-wei; GAO Hui-yuan

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Simultaneous Determination of Six Components in Huangbai by HPLC

更新时间：2024-01-04

- Simultaneous Determination of Six Components in Huangbai by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 2, Pages: 94-97(2013)
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- Published：2013
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LIAN Lian, WAN Guo-sheng, JIA Li-wei, et al. Simultaneous Determination of Six Components in Huangbai by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(2): 94-97.
DOI：

LIAN Lian, WAN Guo-sheng, JIA Li-wei, et al. Simultaneous Determination of Six Components in Huangbai by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(2): 94-97. DOI：

摘要

目的: 建立川黄柏、关黄柏中6种有效成分含量的同时测定方法。方法: 以HPLC采用Diamonsil C18色谱柱 (4.6 mm×250 mm

5 μm)

乙腈-水(1‰乙酸

2mmol醋酸铵)为流动相梯度洗脱

检测波长280 nm

柱温25℃

流速1 mL·min-1。结果: 绿原酸、黄柏碱、木兰花碱、药根碱、巴马汀和小檗碱分别在20.00~320.00

18.75~130.00

25.00~200.00

5.00~100.00

20.00~200.00

0.09~1.80 mg·L-1峰面积和质量浓度线性关系良好(r≥0.999 0)

平均加样回收率分别为98.1%

99.4%

97.5%

97.3%

104.0%

98.5%

RSD分别为0.5%

0.6%

0.8%

1.0%

1.4%

0.9%。结论: 含量测定方法简便、稳定、重复性好

为黄柏药材质量控制和评价提供了依据。

Abstract

Objective: To establish the method for simultaneous determination of 6 active components. Method: Simultaneous determination of chlorogenic acid

phellodendrine

magnoflorine

jatrorrhizine

palmatine and berberine in Huangbai was carried out by HPLC. A Diamonsil C18 (4.6 mm×250 mm

5 μm) column was used. The mobile phase was acetonitrile-water (1‰ acetic acid

2 mmol ammonium acetate) solution in gradient elution. The detection wavelength was set at 280 nm

the column temperature was kept at 25℃ and the flow rate was 1 mL· min-1. Result: The linear range of chlorogenic acid

phellodendrine

magnoflorine

jatrorrhizine

palmatine and berberine was 20.00-320.00

18.75-130.00

25.00-200.00

5.00-100.00

20.00-200.00

0.09-1.80 mg· L-1 separately. The average recovery was 98.1%

99.4%

97.5%

97.3%

104.0%

98.5% separately; and RSD was 0.5%

0.6%

0.8%

1.0%

1.4%

0.9% separately. Conclusion: The method is convenient

stable

reliable and suitable for quality control of Huangbai.

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Related Institution

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

Shaanxi University of Chinese Medicine

The Affiliated Hospital of Shaanxi University of Chinese Medicine

Changzhou Hospital of Traditional Chinese Medicine（TCM）

Xianyang Central Hospital

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