Effect of Huangqin Tang on IL-23/IL-17 Pathway in TNBS-induced Colitis

CHI Hong-gang; ZOU Ying; DAI Shi-xue; ZHENG Xue-bao

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Effect of Huangqin Tang on IL-23/IL-17 Pathway in TNBS-induced Colitis

更新时间：2024-01-04

- Effect of Huangqin Tang on IL-23/IL-17 Pathway in TNBS-induced Colitis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 1, Pages: 211-215(2013)
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- Published：2013
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CHI Hong-gang, ZOU Ying, DAI Shi-xue, et al. Effect of Huangqin Tang on IL-23/IL-17 Pathway in TNBS-induced Colitis[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(1): 211-215.
DOI：

CHI Hong-gang, ZOU Ying, DAI Shi-xue, et al. Effect of Huangqin Tang on IL-23/IL-17 Pathway in TNBS-induced Colitis[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(1): 211-215. DOI：

摘要

目的: 观察黄芩汤对2

6-三硝基苯磺酸(TNBS)诱导的结肠炎大鼠白细胞介素23(IL-23)/白细胞介素17(IL-17)通路的影响。方法: 将32只大鼠随机分为正常对照组、TNBS结肠炎组、TNBS+黄芩汤治疗组(1.2 g·kg-1)、TNBS+美沙拉嗪治疗组(10 mL·kg-1

ig)

每组8只。除正常对照组外

其余各组均应用2

6-三硝基苯磺酸(TNBS)灌肠法建立结肠炎模型

造模完成后

各组分别按设计剂量连续给药1周。用酶联免疫吸附法(ELISA)分析大鼠血清中白细胞介素17(IL-17)、白细胞介素23(IL-23)的水平

用real-time PCR方法检测大鼠结肠组织中IL-17

IL-17R

IL-23

IL-23R

RORγt的mRNA水平。结果: 黄芩汤治疗能有效降低TNBS诱导的结肠炎大鼠血清中IL-17(262.75±17.19 ng·L-1和IL-23(56.75±6.20) ng·L-1的蛋白水平

降低结肠组织中IL-17(2.606±0.8)

IL-17R(5.33±1.10)

IL-23(2.16±0.19)

IL-23R(3.34±0.70)

RORγt(0.74±0.17)的mRNA水平。结论: 黄芩汤能有效的调节TNBS诱导的结肠炎大鼠IL-23/IL-17通路

这可能是其治疗结肠炎的免疫机制之一。

Abstract

Objective:To evaluate the effects of Huangqin Tang on the IL-23/IL-17 pathway in 2

6-trinitrobenzene sulfonic acid(TNBS)-induced colitis model. Method: SD rats were distributed into four group

containing TNBS-induced colitis

Huangqin Tang(1.2 g·kg-1) or mesalazine treatment group(10 mL·kg-1

ig)

negative control with no treatment and the last one was normal sham rats. Except for control group

experimental colitis was induced by rectal instillation of 2

6-trinitrobenzene sulfonic acid (TNBS) into rats. Drugs were given intragastrically for one week in each group continuously according to respectively-designed doses. The protein expressions of IL-17 and IL-23 in serum were determined by ELISA. The mRNA expressions of IL-17

IL-23

IL-17R

IL-23R and RORγt were determined by Real-time PCR. Result: Huangqin Tang markedly attenuated TNBS-induced colitis and effectively inhibited the protein expressions of IL-17(262.75±17.19) ng·L-1 and IL-23(56.75±6.20) ng·L-1. Furthermore

mRNA levels of IL-17(2.606±0.8)

IL-17R(5.33±1.10)

IL-23(2.16±0.19)

IL-23R(3.34±0.70)

RORγt(0.74±0.17)showed a marked reduction after Huangqin Tang teatment. Conclusion: Taken together

our findings demonstrate that Huangqin Tang can exerte therapeutic effects on TNBS-induced colitis

mediate probably by the IL-23/IL-17 pathway.

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