Objective:To study the mechanism of Linggui Zhugan decoction (LGZGD)interference on ventricular remodeling (VR) in rats after acute myocardial infarction (AMI)

through observing the expression of nuclear factor-κB(NF-κB)and NF-κB mRNA of myocardial tissue and the content of NF-κB in ventricular remodeling rats with AMI. Method: AMI model was produced by ligation of coronary artery. 2 weeks after modeling

rats were randomly classified into model

captopril

and low (2.1 g·kg-1)

middle(4.2 g·kg-1) and high (8.4 g·kg-1)dosage of LGZGD group. Control group and the other 5 groups were administered medications intragastrically for 4 consecutive weeks. The content of NF-κB in myocardial tissue and serum was detected by ELISA and the expression of NF-κB was assayed by RT-PCR and the expression of NF-κB mRNA by Western blot. Result: For sham-operation

model

low

middle and large dosage of LGZGD group and captopril group

the content of NF-κB of myocardial tissue was 0.190±0.011

0.772±0.026

0.366±0.059

0.295±0.033

0.235±0.013

0.341±0.023.The relative expression of NF-κB mRNA were 1.000

26.875

6.574

4.340

1.194

5.540.The content of serum NF-κB were (125.85±14.76)

(196.98±17.79)

(163.89±20.08)

(131.73±10.47)

(141.93±10.33)

(133.93±9.27)ng·L-1.The content of NF-κB in serum and the expression of NF-κB

NF-κB mRNA of myocardial tissue of model group increased significantly compared with control group (P＜0.01). Low

middle and high dosage of LGZGD and captopril group could decrease the content of NF-κB in serum and inhibited the expression of NF-κB

NF-κB mRNA in myocardial tissue of model rats significantly compared with model group (P＜0.01 or P＜0.05). Conclusion: The mechanism of LGZGD interference VR of post-AMI seems to be related to the inhibition of NF-κB.