Objective: To evaluate the effects of Banxia Xiexin decocotion on P450 activities by Cocktail probe drugs in rats

provide a reference for clinical medication. Method: Four probe drugs (caffeine

tolbutamide

chlorzoxazone

midazolam) were simultaneously injected to rats after gavaged decoctions of Banxia Xiexin and its compatibilities for 7 days. The plasma concentrations of the four probes were measured by LC-MS-MS and their corresponding pharmacokinetic parameters were calculated. Result: Compared with the control group

the Banxia Xiexin decoction inhibited the espression of CYP2C6

and activated the expression of CYP2E1

but there were no differences on CYP3A1/2

CYP1A2;pungent-swelling group only inhibitied CYP2C6; bitter-descending group inhibitied of CYP1A2 and CYP2C6 expressi on

activatied CYP2E1

CYP3A1/2 no significant difference; sweet-invigorating group inductied the activity of CYP1A2

CYP2E1

inhibition of CYP2C6

and CYP3A1/2 there is no significant difference. Conclusion: Banxia Xiexin decoction and different conbinations have different functions on liver CYP450 enzyme activity.In clinic

We must be aware of possible drug-drug interactions when Banxia Xiexin decoction was used with other drugs.