Objective:A HPLC method was established for simultaneous determination of liensinine

isoliensinine and neferine in Nelumbinis Plumula and the amounts of alkaloids in Nelumbinis Plumula from ten different areas of Fujian province were determined. Method: A Platisil C18 column(4.6 mm×250 mm

5 μm)was used with the mobile phase of acetonitrile-water(55∶45)(adjusted pH to 9.8 using ethanediamine) at detection wavelength of 282 nm.The flow rate was 1.0 mL·min-1. Result: The linear ranges of liensinine

isoliensinine and neferine were 3.9-115.9 mg·L-1(r=0.999 8)

4.0-120.7 mg·L-1(r=0.999 8)

9.7-290.2 mg·L-1(r=0.999 8) respectively. The average recoveries were 100.24%

99.17%

100.10%

and RSD were 0.78%

1.31%

1.40% respectively. Most of the amounts of total alkaloids in Nelumbinis Plumula from ten different areas of Fujian Province were 1.50-2.00%. Conclusion: The method is simple

sensitive

accurate and reliable for the determination of three alkaloids in Nelumbinis Plumula. The amount of total alkaloids in Nelumbinis Plumula named Jihe from Jianning was 2.28% and it was the highest in the ten samples.