Effect of Huoxue Jiedu Formula on Endothelin-1 in Retina of Diabetic Rats

YAO Qing; HAN Jing; HUANG Li-ming; YU Jun-da; WANG Wei

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Effect of Huoxue Jiedu Formula on Endothelin-1 in Retina of Diabetic Rats

更新时间：2024-01-04

- Effect of Huoxue Jiedu Formula on Endothelin-1 in Retina of Diabetic Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 20, Pages: 169-172(2012)
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- Published：2012
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YAO Qing, HAN Jing, HUANG Li-ming, et al. Effect of Huoxue Jiedu Formula on Endothelin-1 in Retina of Diabetic Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(20): 169-172.
DOI：

YAO Qing, HAN Jing, HUANG Li-ming, et al. Effect of Huoxue Jiedu Formula on Endothelin-1 in Retina of Diabetic Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(20): 169-172. DOI：

摘要

目的: 探讨活血解毒方对糖尿病大鼠视网膜病变血管紧张素Ⅱ(angiotensinⅡ

AngⅡ)和内皮素-1(endothelin-1

ET-1)的影响。方法: 采用链脲佐菌素(65 mg·kg-1)1次性ip的方法建立糖尿病大鼠模型

随机分为模型组和活血解毒方高、中、低剂量组(15.4

7.70

3.85 g·kg-1)及导升明组(0.167 g·kg-1)

ig给药

20周后处死

取血清检测AngⅡ和ET-1的含量。摘除眼球应用免疫组织化学标记抗生蛋白连菌素法(LSAB)法检测大鼠视网膜全层ET-1的表达。取大鼠视网膜组织

采用实时聚合酶链反应Real-time PCR检测ET-1 mRNA的表达。结果: 糖尿病模型组大鼠血清AngⅡ和ET-1含量分别为(417.95±156.11)

(330.12±81.59) ng·L-1

比正常组升高(P＜0.01)。糖尿病模型组大鼠视网膜LSAB法标记染色病理切片中ET-1蛋白的表达升高

积分吸光度(IA)为(53.45±15.67)

与正常组相比(12.76±3.27)显著升高(P＜0.01)

活血解毒方各剂量组及导升明组与模型组相比显著降低(P＜0.01)。模型组ET-1 mRNA的表达显著高于正常组

活血解毒方各剂量组的表达明显低于模型组

与模型组相比有显著性差异。结论: 活血解毒方可能通过降低视网膜ET-1的表达

延缓糖尿病大鼠视网膜病变的发展。

Abstract

Objective:To observe the effect of Huoxue Jiedu formula on angiotensin Ⅱ(Ang Ⅱ) and endothelin-1(ET-1) in retina of diabetic rats. Method: A single intraperitoneal injection of streptozotocin(65 mg·kg-1)was used to induce diabetic rats. Model rats were randomly divided into diabetes mellitus(DM)group

Huoxue Jiedu formula groups(15.4

7.70

3.85 g·kg-1) and Doxium group(0.167 g·kg-1).The normal group and model group were given normal water by intragastric administration. All rats were killed after 20 weeks. The serum AngⅡ and ET-1 levels were measured. Immunological histochemistry assay was used to observe the express of ET-1.The expressive abundance of ET-1mRNA in rat's retina were detected by real-time quantitative PCR. Result: The levels of AngⅡ and ET-1 in serum of model group were (417.95±156.11)

(330.12±81.59) ng·L-1

which was higher than control group.The retinal ET-1 protein expression and ET-1mRNA were significantly higher in DM rats than in control group(P＜0.01).The levels of ET-1 in serum of Huoxue Jiedu formula groups were lower than those in model group(P＜0.01).Compared with the model group

the expression of ET-1 in Huoxue Jiedu formula groups depressed evidently(P＜0.01)and the expression of ET-1mRNA were reduced than those in model groups(P＜0.01). Conclusion: Huoxue Jiedu formula medicine can reduce the expression of ET-1 in retina of diabetic rats.

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Related Author

YAO Qing1

HAN Jing1

HUANG Li-ming1

YU Jun-da1

WANG Wei1

XIE Huiyi

CHEN Junran

HUANG Boning

Related Institution

Beijing University of Chinese Medicine

Ningxia Medical University

Hu Tianbao Community-Level Old Chinese Medicine Studio

State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs， Institute of Chinese Materia Medica， China Academy of Chinese Medical Sciences

The First Affiliated Hospital of Guangzhou University of Chinese Medicine， Guangdong Clinical Research Academy of Chinese Medicine， Guangdong Engineering Research Center of Commercialization of Medical Institution Preparations and Traditional Chinese Medicines， Guangdong Engineering Technology Research Center of Commercialization of Linnan Special Medical Institution Preparations

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