Study on HPLC Fingerprint Analysis of Formula Granules
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Study on HPLC Fingerprint Analysis of Formula Granules
Chinese Journal of Experimental Traditional Medical FormulaeVol. 19, Issue 2, Pages: 81-84(2013)
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Published:2013
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XU Ai-li, DONG Yu-juan. Study on HPLC Fingerprint Analysis of Formula Granules[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(2): 81-84.
DOI:
XU Ai-li, DONG Yu-juan. Study on HPLC Fingerprint Analysis of Formula Granules[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(2): 81-84.DOI:
Study on HPLC Fingerprint Analysis of Formula Granules
Objective: To establish a method of fingerprint analysis on S. chinensis Formula Granules by HPLC and provide a reference for quality evaluation of S. chinensis Formula Granules. Method: The chromatographic fingerprints were obtained by the Waters Xbridge C18 column(4.6 mm×250 mm
5 μm) with the gradient elution solvent system composed of acetonitrile-water. The detective wavelength was set at 254 nm. The flow rate was 1.0 mL· min-1 and the column temperature was maintained at 25℃. Result: 18 co-possessing peaks were selected as the fingerprint peaks of S. chinensis Formula Granules and good similarities with correlation coefficients higher than 0.9 were found in fingerprints between the different samples of S. chinensis Formula Granules and the standard fingerprint. Conclusion: The method with good reproducibility is simple and accurate. It can be used as a quality control method for S. chinensis Formula Granules.