Determination of Psoralen and Isopsoralen in LBSP by HPLC

ZHANG Rui; FENG Xiao-chuan; XU Yan-zhao

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Determination of Psoralen and Isopsoralen in LBSP by HPLC

更新时间：2024-01-04

- Determination of Psoralen and Isopsoralen in LBSP by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 21, Pages: 142-144(2012)
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- Published：2012
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ZHANG Rui, FENG Xiao-chuan, XU Yan-zhao. Determination of Psoralen and Isopsoralen in LBSP by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(21): 142-144.
DOI：

ZHANG Rui, FENG Xiao-chuan, XU Yan-zhao. Determination of Psoralen and Isopsoralen in LBSP by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(21): 142-144. DOI：

摘要

目的: 应用高效液相色谱法测定癃闭舒片(LBSP)中补骨脂素和异补骨脂素的含量。方法: C18色谱柱(4.6 mm×150 mm

5 μm)

甲醇-0.02 mol ·L-1磷酸二氢钾水溶液(45:55)为流动相

流速1.0 mL ·min-1

检测波长246 nm。结果: 补骨脂素在0.144 0~0.720 0 μg进样量与峰面积呈良好的线性关系(r=0.999 8)

平均加样回收率为99.16%

RSD 1.21%(n=6);异补骨脂素在0.136 2~0.681 0 μg进样量与峰面积呈良好的线性关系(r=0.999 8)

平均加样回收率为98.52%

RSD 1.58%(n=6)。结论: 该方法测定结果准确、灵敏、重复性好。

Abstract

Objective: To develop an HPLC method for determination of the content of psoralen and isopsoralen in Longbishu pills(LBSP). Method: An C18 column was used as the chromatographic column.The mobile phase consisted of methanol-0.02 mol·L-1 Sodium Dihydrogen Phosphate Solution (45: 55)

the flow rate was 1.0 mL ·min-1.The UV detection wavelength was at 246 nm. Result: There was a good linear relationship between the concentration of psoralen and peak area value when the concentrations of psoralen was within the range of 0.144 0-0.720 0 μg(r=0.999 8).The average recovery was 99.16%

RSD 1.21%(n=6).There was a good linear relationship between the concentration of isopsoralen and peak area value when the concentrations of isopsoralen was within the range of 0.136 2-0.681 0 μg(r=0.999 8).The average recovery was 98.52%

RSD 1.58%(n=6). Conclusion: The method was accurate

sensitive

reproducible and may be used in the determination of psoralen and isopsoralen in LBSP.

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